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Sertoli cell differentiation in rhesus monkey (Macaca mulatta) is an early event in puberty and precedes attainment of the adult complement of ndifferentiated permatogonia.
MedLine Citation:
PMID:  22232743     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In primates, the time course of Sertoli cell proliferation and differentiation during puberty and its relationship to the expansion of undifferentiated Type A spermatogonia that occurs at this critical stage of development is poorly defined. Mid and late juvenile and early and late pubertal male rhesus monkeys were studied. Testes were immersion fixed, embedded in paraffin and sectioned at 5 um. Sertoli cell number per testis, S-phase labeling (BrdU) and growth fraction (Ki67 labeling) were determined and correlated with corresponding parameters for undifferentiated Type A spermatogonia (A dark and A pale). Dual label fluorescence was used in addition to histochemistry to monitor spermatogonial differentiation during the peripubertal period using GFRα-1 and cKIT as markers. While the adult complement of Sertoli cells/testis was attained in Early Pubertal monkeys after only a few weeks of exposure to the elevated gonadotrophin secretion characteristic of this developmental stage, the number of undifferentiated Type A spermatogonia several months later in Mid Pubertal monkeys was only 50% of that in adult testes. Both A dark and A pale spermatogonia exhibited high S-phase BrdU labeling at all stages of juvenile and pubertal development. Spermatogonial differentiation, as reflected histochemically and by relative changes in GFRα-1 and cKIT expression, were not observed until after the initiation of puberty. In the rhesus monkey and probably other higher primates including man, the pubertal proliferation of undifferentiated spermatogonia is insidious and proceeds in the wake of a surge in Sertoli cell proliferation following termination of the juvenile stage of development.
Authors:
David R Simorangkir; Suresh Ramaswamy; Gary R Marshall; Rachel M Roslund; Tony Plant
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-9
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
D Simorangkir, Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, United States.
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