Document Detail

Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
MedLine Citation:
PMID:  21318592     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.
METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative quantification real-time RT-PCR to determine the efficiency of germ cell differentiation.
RESULTS: VUB hESC lines can differentiate spontaneously towards the germ cell lineage, however, more consistently in an embryoid body approach than in monolayer cultures. BMPs and SCCM significantly improve VASA expression, but do not have a synergistic effect. Direct contact of differentiating hESC with Sertoli cells does not improve VASA expression.
CONCLUSIONS: SCCM contains inductive factors for germ cell differentiation and could represent an element for in-vitro differentiation to germ cells.
Mieke Geens; Karen Dora Sermon; Hilde Van de Velde; Herman Tournaye
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-12
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  28     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-08-08     Completed Date:  2012-02-17     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  471-80     Citation Subset:  IM    
Research Group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
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MeSH Terms
Cell Differentiation*
Cell Lineage
Culture Media, Conditioned
Embryonic Stem Cells / cytology*,  metabolism
Germ Cells / cytology*,  metabolism
Sertoli Cells / cytology*,  metabolism
Reg. No./Substance:
0/Culture Media, Conditioned

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