| Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells. | |
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MedLine Citation:
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PMID: 21318592 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols. METHODS: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative quantification real-time RT-PCR to determine the efficiency of germ cell differentiation. RESULTS: VUB hESC lines can differentiate spontaneously towards the germ cell lineage, however, more consistently in an embryoid body approach than in monolayer cultures. BMPs and SCCM significantly improve VASA expression, but do not have a synergistic effect. Direct contact of differentiating hESC with Sertoli cells does not improve VASA expression. CONCLUSIONS: SCCM contains inductive factors for germ cell differentiation and could represent an element for in-vitro differentiation to germ cells. |
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Authors:
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Mieke Geens; Karen Dora Sermon; Hilde Van de Velde; Herman Tournaye |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-02-12 |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 28 ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-08-08 Completed Date: 2012-02-17 Revised Date: 2013-05-26 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: Netherlands |
Other Details:
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Languages: eng Pagination: 471-80 Citation Subset: IM |
Affiliation:
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Research Group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium. Mieke.Geens@uzbrussel.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation* Cell Lineage Culture Media, Conditioned Embryonic Stem Cells / cytology*, metabolism Germ Cells / cytology*, metabolism Humans Male Mice Sertoli Cells / cytology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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