Document Detail

Sertoli cell-conditioned medium affects nucleoside utilization in vitro.
MedLine Citation:
PMID:  8056634     Owner:  NLM     Status:  MEDLINE    
Rat Sertoli cell-conditioned medium (SCCM) or low molecular weight filtrate of SCCM (< 1,000 Da molecular weight) inhibited the uptake of [3H]thymidine into cells in culture; [3H]thymidine is incorporated into DNA by means of a salvage pathway. The incorporation of radioactivity into DNA from [14C]N5,N10-methylene-tetrahydrofolate, required for the thymidylate synthetase reaction, was not inhibited by SCCM and reflected the increase in cell number. SCCM specifically inhibited the incorporation of pyrimidines ([3H]uracil, [3H]thymidine) with no effect on the transport or incorporation of [3H]adenosine. Inhibition of thymidine uptake by SCCM could have occurred by a direct competition, i.e., the secretion of thymidine into the medium by the Sertoli cells, or by an indirect mechanism that would result in an inhibition of transport. The activity was partially purified by membrane ultrafiltration, ion exchange chromatography, and sequential extraction. Addition of SCCM filtrate (< 1,000 Da molecular weight) to growth-arrested cells cultured in the presence of inhibitors of dihydrofolate reductase resulted in cell proliferation, suggesting that the factor is involved in thymidine biosynthesis. This activity may play a role in the regulation of nucleoside biosynthesis and/or utilization in the testis.
D J Lamb; S Shubhada; K Baker; S K Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of andrology     Volume:  15     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1994 Mar-Apr
Date Detail:
Created Date:  1994-09-12     Completed Date:  1994-09-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-24     Citation Subset:  IM    
Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
3T3 Cells
Biological Factors / isolation & purification,  metabolism*,  secretion
Biological Transport
Cell Division
Cell Line
Culture Media, Conditioned
DNA / biosynthesis
Fluorouracil / pharmacology
Nucleosides / metabolism*
Rats, Sprague-Dawley
Sertoli Cells / drug effects,  metabolism*,  secretion
Thymidine / metabolism,  secretion
Grant Support
Reg. No./Substance:
0/Biological Factors; 0/Culture Media, Conditioned; 0/Nucleosides; 50-89-5/Thymidine; 51-21-8/Fluorouracil; 9007-49-2/DNA

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