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Serotonin and its 5-HT1 receptor in human mastocytosis.
MedLine Citation:
PMID:  22268664     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Context: Human mastocytosis is a rare disease, in which the serotonergic system may be involved. Objective: The objective of the present study was to examine the possible presence of serotonin (5-HT) and its 5-HT1A receptor (R) in the skin of patients with mastocytosis. In addition, the effect of the 5-HT1AR was tested on human mastocytosis cells, cultured in vitro. Materials and methods: The expression of 5-HT and 5-HT1AR in patients with urticaria pigmentosa and mastocytoma was studied using immunohistochemistry. The effects of 8-OH-DPAT, an agonist of 5-HT1AR, on the proliferation (cell number), viability, apoptosis, spontaneous release of histamine, as well as a possible 5-HT metabolism, in the human HMC-1 mast cell line, were investigated. Results: Both 5-HT and 5-HT1AR were expressed in the mast cells in biopsies of mastocytoma and urticaria pigmentosa, as well as in HMC-1 cells. However, no metabolism of 5-HT by the cell line could be detected by the methodology used. The 5-HT1AR agonist had no significant effect on the viability and number of HMC-1 cells, and was without effect on the apoptosis. At concentrations of 10(-6) mol/L and 10(-8) -10(-10) mol/L (i.e. also at physiological concentrations), the agonist inhibited histamine release by these cells by as much as 30%. Conclusion: These findings indicate that 5-HT and its 5-HT1AR are expressed in human mastocytosis and that an agonist of the 5-HT1AR might be of value in the treatment of these patients.
Authors:
Markus Ritter; Husameldin El-Nour; Mari-Anne Hedblad; Joseph H Butterfield; Olof Beck; Niclaus Stephanson; Mikael Holst; Ricardo Giscombe; Efraim C Azmitia; Klas Nordlind
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-23
Journal Detail:
Title:  Immunopharmacology and immunotoxicology     Volume:  -     ISSN:  1532-2513     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8800150     Medline TA:  Immunopharmacol Immunotoxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Dermatology and Venereology Unit, Department of Medicine, Karolinska Institutet, Karolinska University Hospital , Solna, Stockholm Sweden.
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