Document Detail


Serotonin inhibits outgrowth of goldfish retina and impairs the trophic effect of taurine.
MedLine Citation:
PMID:  7523693     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The regeneration of explants prepared from goldfish retinas with a prior crush of the optic nerve is stimulated by the sulphur amino acid, taurine. Serotonin has been reported to modify survival, proliferation, and outgrowth of nervous tissue. In the present work we evaluated the effect of serotonin and some serotonergic agonists on the neuritic outgrowth from goldfish retinal explants. Serotonin, its precursor, 5-hydroxytryptophan, and the 5HT1A receptor agonists, 8-hydroxy-2-(di-n-propylamino)tetralin and buspirone, inhibited the outgrowth. The blockers of serotonin uptake, imipramine and citalopram, were also inhibitors of neurite sprouting. Imipramine favoured the inhibitory effect of serotonin at 10 days in culture. The concentration of serotonin and its metabolite, 5-hydroxyindoleacetic acid, decreased in the retina at 3 and 5 days after the crush of the optic nerve. Serotonin levels started to recover after 5 days post-lesion, and the metabolite also increased. This indicates that the lesion increases the turnover rate of serotonin and this may be related to its role in regeneration. Serotonin concentration was elevated by the intraocular administration of its precursor, 5-hydroxytryptophan, indicating that the capacity for synthesis was preserved after the crush, but that it was smaller in the post-lesioned retinas. The trophic effect of taurine was impaired by a low concentration of serotonin, probably by opposing the final effect on growth via different targets. These results support a role of serotonin in the regeneration of goldfish retina probably through 5HT1A receptors.
Authors:
L Lima; P Matus; M Urbina
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  38     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-11-23     Completed Date:  1994-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  444-50     Citation Subset:  IM    
Affiliation:
Laboratorio de Neuroquímica, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
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MeSH Terms
Descriptor/Qualifier:
5-Hydroxytryptophan / metabolism
Animals
Goldfish / physiology*
Hydroxyindoleacetic Acid / metabolism
Nerve Crush
Nerve Regeneration / drug effects
Neurites / drug effects
Optic Nerve / drug effects,  physiology
Receptors, Serotonin / drug effects
Retina / drug effects,  growth & development*,  metabolism
Serotonin / biosynthesis,  metabolism,  pharmacology*
Serotonin Agonists / pharmacology
Serotonin Uptake Inhibitors / pharmacology
Taurine / antagonists & inhibitors*,  pharmacology
Chemical
Reg. No./Substance:
0/Receptors, Serotonin; 0/Serotonin Agonists; 0/Serotonin Uptake Inhibitors; 107-35-7/Taurine; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 56-69-9/5-Hydroxytryptophan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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