Document Detail


Serotonin inhibits low-threshold spike interneurons in the striatum.
MedLine Citation:
PMID:  22495583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Low-threshold spike interneurons (LTSIs) are important elements of the striatal architecture and the only known source of nitric oxide in this nucleus, but their rarity has so far prevented systematic studies. Here, we used transgenic mice in which green fluorescent protein is expressed under control of the neuropeptide Y (NPY) promoter and striatal NPY-expressing LTSIs can be easily identified, to investigate the effects of serotonin on these neurons. In sharp contrast with its excitatory action on other striatal interneurons, serotonin (30 μM) strongly inhibited LTSIs, reducing or abolishing their spontaneous firing activity and causing membrane hyperpolarisations.These hyperpolarisations persisted in the presence of tetrodotoxin, were mimicked by 5-HT(2C) receptor agonists and reversed by 5-HT(2C) antagonists. Voltage-clamp slow-ramp experiments showed that serotonin caused a strong increase in an outward current activated by depolarisations that was blocked by the specific M current blocker XE 991. In current-clamp experiments,XE 991 per se caused membrane depolarisations in LTSIs and subsequent application of serotonin (in the presence of XE 991) failed to affect these neurons.We concluded that serotonin strongly inhibits striatal LTSIs acting through postsynaptic 5-HT(2C) receptors and increasing an M type current.
Authors:
Sarah Cains; Craig P Blomeley; Enrico Bracci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-10
Journal Detail:
Title:  The Journal of physiology     Volume:  590     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-10-25     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2241-52     Citation Subset:  IM    
Affiliation:
Life Sciences, University of Manchester, AV Hill Building, Oxford Road, Manchester M13 9PT, UK.
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MeSH Terms
Descriptor/Qualifier:
Androstadienes / pharmacology
Animals
Anthracenes / pharmacology
Corpus Striatum / physiology*
Female
Interneurons / physiology*
Male
Mice
Mice, Transgenic
Nitric Oxide / physiology
Potassium Channel Blockers / pharmacology
Rats
Rats, Sprague-Dawley
Serotonin / physiology*
Serotonin Antagonists / pharmacology
Grant Support
ID/Acronym/Agency:
084706/Z/08/Z//Wellcome Trust
Chemical
Reg. No./Substance:
0/10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone; 0/Androstadienes; 0/Anthracenes; 0/Potassium Channel Blockers; 0/Serotonin Antagonists; 10102-43-9/Nitric Oxide; 19545-26-7/wortmannin; 50-67-9/Serotonin
Comments/Corrections

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