| Serology of culture-confirmed cases of human granulocytic ehrlichiosis. | |
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MedLine Citation:
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PMID: 10655359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We evaluated the antibody responses in the sera of 24 patients with culture-confirmed human granulocytic ehrlichiosis (HGE). Antibody titers were measured by an indirect immunofluorescent-antibody assay (IFA) by using a local human isolate as the source of antigen. All patients received appropriate antimicrobial treatment. One hundred five serum specimens collected at baseline and at periodic intervals for up to 14 months were included in the study. Seroconversion was observed in 21 of 23 patients (91.3%) from whom convalescent-phase sera were obtained. Antibodies were first detected at an average of 11.5 days after onset of symptoms. Peak titers (>/=2,560 for 71.4% of patients and >/=640 for 95.2% of patients) were obtained an average of 14.7 days after onset of symptoms. Eleven of 13 patients (84.6%) from whom sera were collected between 6 and 10 months after onset of symptoms were still seropositive, and sera from 5 of 10 (50%) patients tested positive between 11 and 14 months after onset of symptoms. For a subset of 71 serum specimens from 17 patients with culture-confirmed HGE also tested by IFA by using either a human isolate from Wisconsin or an Ehrlichia equi isolate from a horse, there was qualitative agreement for 62 serum specimens (87. 3%). Peak titers were higher, however, with the local human HGE isolate, but the difference was not statistically significant. In summary, most patients with culture-confirmed HGE develop antibodies within 2 weeks of onset of symptoms. Antibodies reach high titers during the first month and remain detectable in about one-half of patients at 1 year after onset of symptoms. |
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Authors:
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M E Aguero-Rosenfeld; F Kalantarpour; M Baluch; H W Horowitz; D F McKenna; J T Raffalli; T c Hsieh; J Wu; J S Dumler; G P Wormser |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 38 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-03-16 Completed Date: 2000-03-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 635-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, New York 10505, USA. maria_aguero-rosenfeld@nymc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Antibodies, Bacterial / blood* Antigens, Bacterial / immunology Ehrlichia / immunology*, isolation & purification Ehrlichiosis / diagnosis*, microbiology Female Fluorescent Antibody Technique, Indirect Granulocytes / microbiology Humans Infant, Newborn Male Middle Aged |
| Grant Support | |
ID/Acronym/Agency:
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R01AI41213/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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