Document Detail


Serology of culture-confirmed cases of human granulocytic ehrlichiosis.
MedLine Citation:
PMID:  10655359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We evaluated the antibody responses in the sera of 24 patients with culture-confirmed human granulocytic ehrlichiosis (HGE). Antibody titers were measured by an indirect immunofluorescent-antibody assay (IFA) by using a local human isolate as the source of antigen. All patients received appropriate antimicrobial treatment. One hundred five serum specimens collected at baseline and at periodic intervals for up to 14 months were included in the study. Seroconversion was observed in 21 of 23 patients (91.3%) from whom convalescent-phase sera were obtained. Antibodies were first detected at an average of 11.5 days after onset of symptoms. Peak titers (>/=2,560 for 71.4% of patients and >/=640 for 95.2% of patients) were obtained an average of 14.7 days after onset of symptoms. Eleven of 13 patients (84.6%) from whom sera were collected between 6 and 10 months after onset of symptoms were still seropositive, and sera from 5 of 10 (50%) patients tested positive between 11 and 14 months after onset of symptoms. For a subset of 71 serum specimens from 17 patients with culture-confirmed HGE also tested by IFA by using either a human isolate from Wisconsin or an Ehrlichia equi isolate from a horse, there was qualitative agreement for 62 serum specimens (87. 3%). Peak titers were higher, however, with the local human HGE isolate, but the difference was not statistically significant. In summary, most patients with culture-confirmed HGE develop antibodies within 2 weeks of onset of symptoms. Antibodies reach high titers during the first month and remain detectable in about one-half of patients at 1 year after onset of symptoms.
Authors:
M E Aguero-Rosenfeld; F Kalantarpour; M Baluch; H W Horowitz; D F McKenna; J T Raffalli; T c Hsieh; J Wu; J S Dumler; G P Wormser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  38     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-16     Completed Date:  2000-03-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  635-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, New York Medical College, Westchester Medical Center, Valhalla, New York 10505, USA. maria_aguero-rosenfeld@nymc.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antibodies, Bacterial / blood*
Antigens, Bacterial / immunology
Ehrlichia / immunology*,  isolation & purification
Ehrlichiosis / diagnosis*,  microbiology
Female
Fluorescent Antibody Technique, Indirect
Granulocytes / microbiology
Humans
Infant, Newborn
Male
Middle Aged
Grant Support
ID/Acronym/Agency:
R01AI41213/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial
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