Document Detail

Serological and virological investigation of the role of the herpesviruses EBV, CMV and HHV-6 in post-infective fatigue syndrome.
MedLine Citation:
PMID:  20827765     Owner:  NLM     Status:  MEDLINE    
Multiple previous studies have sought evidence for ongoing, active infection with, or reactivation of, Herpesviruses in patients with chronic fatigue syndrome (CFS), with conflicting results. This study aimed to clarify this by studying 20 patients enrolled in a well-characterized model of the onset and evolution of CFS, the prospective cohort of the Dubbo Infection Outcomes Study (DIOS). The patients selected for examination included five CFS patients with primary Epstein-Barr virus (EBV) infection; five CFS patients with acute viral infection not caused by EBV; and 10 matched controls with prompt resolution of primary EBV infection. Serum samples from three timepoints were assayed using a comprehensive range of serological assays for EBV, HHV-6, and CMV. Viral genomes were assessed using quantitative PCR assays. All patients were seropositive for HHV-6, and 10 were seropositive for CMV at infection baseline (five patients and five controls). Low titer CMV IgM antibodies were found at infection baseline in two of these cases and three control patients. HHV-6 IgG antibody titers were highest at infection baseline but did not differ between the CFS cases and the control patients. There were increases in EBV IgG VCA p18, EBNA-1 IgG, and EA IgG titers over time, but these did not differ between CFS cases and control patients. EBV and HHV6 DNA levels were at control levels in a minority of samples, and CMV was undetectable in all samples. These data do not support the hypothesis of ongoing or reactivated EBV, HHV-6, or CMV infection in the pathogenesis of CFS.
Barbara Cameron; Louis Flamand; Hedy Juwana; Jaap Middeldorp; Zin Naing; William Rawlinson; Dharam Ablashi; Andrew Lloyd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medical virology     Volume:  82     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-09     Completed Date:  2010-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1684-8     Citation Subset:  IM    
Center for Infection and Inflammation Research, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
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MeSH Terms
Antibodies, Viral / blood
Cytomegalovirus / genetics,  immunology,  isolation & purification*
DNA, Viral / genetics,  isolation & purification
Fatigue Syndrome, Chronic / virology*
Herpesvirus 4, Human / genetics,  immunology,  isolation & purification*
Herpesvirus 6, Human / genetics,  immunology,  isolation & purification*
Immunoenzyme Techniques
Immunoglobulin G / blood
Middle Aged
Polymerase Chain Reaction
Young Adult
Grant Support
U50/CCU019851-01//PHS HHS
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA, Viral; 0/Immunoglobulin G

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