Document Detail


Serological response to plasmid-encoded antigens in children and adults with shigellosis.
MedLine Citation:
PMID:  3066570     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The immunological response to plasmid-encoded antigens of virulent Shigella was determined in Thai children less than 4 yr of age and in Thai adults by immunoblot analysis and ELISA. Forty-two percent (8/19) of Thai children and 4% (1/22) of Thai adults with shigellosis developed a greater than or equal to 4-fold rise in IgG antibody titer to water-extracted antigens of Shigella flexneri M90T by ELISA (p = 0.006). Two children and one lactating mother with shigellosis developed a 4-fold rise in serum IgA antibody titers to water-extracted antigens of M90T. The results of the ELISA were confirmed by immunoblot analysis in all of the 41 paired sera examined. Five patients developed IgA, and four developed IgM, antibodies as detected by immunoblot analysis, that were not detected by ELISA. The reciprocal log2 geometric mean titers of antibodies to plasmid-encoded antigens in acute sera was higher in Thai adults than Thai children: IgG 7,265 versus 1,659; IgM 879 versus 480; and IgA 662 versus 60 (p less than 0.001). Thai adults had high titers of antibodies to plasmid-encoded antigens in their acute sera, but were susceptible to Shigella infections, although they were historically less susceptible than Thai children.
Authors:
P Echeverria; S Hanchalay; D N Taylor
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  10     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM    
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Bacterial / analysis*
Antigens, Bacterial / immunology*
Child
Disease Susceptibility
Dysentery, Bacillary / immunology*
Enzyme-Linked Immunosorbent Assay
Humans
Immunoblotting
Immunoglobulin A / analysis
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Plasmids
Shigella flexneri / immunology
Thailand
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Primary lymphocutaneous Nocardia brasiliensis infection: three case reports and a review of the lite...
Next Document:  Results of a prospective, 18-month clinical evaluation of culture, cytotoxin testing, and culturette...