| Serological response to Helicobacter pylori recombinant antigens in Chilean infected patients with duodenal ulcer, non-ulcer dyspepsia and gastric cancer. | |
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MedLine Citation:
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PMID: 10660136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously cloned 10 Helicobacter pylori antigen genes from a Chilean strain including: cytotoxin VacA, a truncated region of CagA (called A17), a species-specific protein (Ag26), urease subunits (UreA, UreB), a flagellin, (FlaB), heat shock proteins (HspA and HspB), an adhesin (HpaA) and a lipoprotein (Lpp20). Immunogenicity of these antigens was tested by immunoblot with sera of Chilean infected patients, revealing that HpaA, A17, HspB and VacA were more frequently recognized (86%, 82%, 68% and 68%, respectively). According to the clinical condition, it was determined that Lpp20 was preferentially recognized by sera from non-ulcer dyspepsia patients (80%), A17 and VacA by patients with duodenal ulcer (92% and 83% respectively), and HspB by patients with duodenal ulcer (83%) and gastric cancer (90%). An ELISA was developed with a purified mixture of A17 and VacA antigens to test the different groups of patients. It was found that sera from duodenal ulcer patients showed higher values than those from non-ulcer dyspepsia patients, but this difference was not significant (p<0.2). Moreover, sera from gastric cancer patients showed values lower than those from non-ulcer dyspepsia patients (p<0.019). These results indicate that, in the Chilean population, antibodies raised against VacA and A 7 are not markers either for duodenal ulcer or for gastric cancer. |
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Authors:
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P Opazo; I Müller; A Rollán; P Valenzuela; A Yudelevich; R García-de la Guarda; S Urra; A Venegas |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 107 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-14 Completed Date: 2000-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
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Languages: eng Pagination: 1069-78 Citation Subset: IM |
Affiliation:
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Unidad de Biología Molecular, BIOS Chile IGSA, Santiago. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antibodies, Bacterial / blood* Antigens, Bacterial* / genetics Bacterial Proteins / genetics, immunology Biological Markers Case-Control Studies Chile Duodenal Ulcer / complications, immunology* Dyspepsia / complications, immunology* Enzyme-Linked Immunosorbent Assay Escherichia coli / genetics Genes, Bacterial Heat-Shock Proteins / genetics, immunology Helicobacter Infections / complications, immunology Helicobacter pylori / genetics, immunology* Humans Recombinant Proteins / genetics, immunology Stomach Neoplasms / complications, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Biological Markers; 0/Heat-Shock Proteins; 0/HspA protein, bacteria; 0/HspB protein, Helicobacter pylori; 0/Recombinant Proteins; 0/VacA protein, Helicobacter pylori |
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