Document Detail

Serological response to Helicobacter pylori recombinant antigens in Chilean infected patients with duodenal ulcer, non-ulcer dyspepsia and gastric cancer.
MedLine Citation:
PMID:  10660136     Owner:  NLM     Status:  MEDLINE    
We have previously cloned 10 Helicobacter pylori antigen genes from a Chilean strain including: cytotoxin VacA, a truncated region of CagA (called A17), a species-specific protein (Ag26), urease subunits (UreA, UreB), a flagellin, (FlaB), heat shock proteins (HspA and HspB), an adhesin (HpaA) and a lipoprotein (Lpp20). Immunogenicity of these antigens was tested by immunoblot with sera of Chilean infected patients, revealing that HpaA, A17, HspB and VacA were more frequently recognized (86%, 82%, 68% and 68%, respectively). According to the clinical condition, it was determined that Lpp20 was preferentially recognized by sera from non-ulcer dyspepsia patients (80%), A17 and VacA by patients with duodenal ulcer (92% and 83% respectively), and HspB by patients with duodenal ulcer (83%) and gastric cancer (90%). An ELISA was developed with a purified mixture of A17 and VacA antigens to test the different groups of patients. It was found that sera from duodenal ulcer patients showed higher values than those from non-ulcer dyspepsia patients, but this difference was not significant (p<0.2). Moreover, sera from gastric cancer patients showed values lower than those from non-ulcer dyspepsia patients (p<0.019). These results indicate that, in the Chilean population, antibodies raised against VacA and A 7 are not markers either for duodenal ulcer or for gastric cancer.
P Opazo; I Müller; A Rollán; P Valenzuela; A Yudelevich; R García-de la Guarda; S Urra; A Venegas
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  107     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-14     Completed Date:  2000-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  1069-78     Citation Subset:  IM    
Unidad de Biología Molecular, BIOS Chile IGSA, Santiago.
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MeSH Terms
Antibodies, Bacterial / blood*
Antigens, Bacterial* / genetics
Bacterial Proteins / genetics,  immunology
Biological Markers
Case-Control Studies
Duodenal Ulcer / complications,  immunology*
Dyspepsia / complications,  immunology*
Enzyme-Linked Immunosorbent Assay
Escherichia coli / genetics
Genes, Bacterial
Heat-Shock Proteins / genetics,  immunology
Helicobacter Infections / complications,  immunology
Helicobacter pylori / genetics,  immunology*
Recombinant Proteins / genetics,  immunology
Stomach Neoplasms / complications,  immunology*
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Biological Markers; 0/Heat-Shock Proteins; 0/HspA protein, bacteria; 0/HspB protein, Helicobacter pylori; 0/Recombinant Proteins; 0/VacA protein, Helicobacter pylori

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