| Serological identification and separation of canine lymphocyte subpopulations. | |
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MedLine Citation:
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PMID: 6210205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro treatment of bone marrow grafts with absorbed rabbit-antidog thymocyte globulin (ATG) prevented graft-versus-host disease in a substantial number of the dogs differing by one DLA haplotype. Absorbed ATG has now been used for serological identification of canine lymphocyte populations. Specific labeling of canine T-lymphocytes by absorbed ATG could be demonstrated by (a) a distribution of ATG-positive cells in suspensions of canine lymphoid organs similar to that of T cells observed in other species and by specific staining of paracortical thymus-dependent lymph node areas in immunohistochemistry, (b) complementary labeling of nylon-wool-separated cells by ATG and antiimmunoglobulin sera, and (c) correlation of ATG surface labeling with responder activities in mixed lymphocyte cultures. |
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Authors:
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E Holler; H J Kolb; U Bodenberger; R Eckstein; G Hoffmann-Fezer; R P Linke; W Mempel; H Rodt; S Thierfelder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental hematology Volume: 12 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 1984 Nov |
Date Detail:
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Created Date: 1985-02-04 Completed Date: 1985-02-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 774-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antilymphocyte Serum / immunology* Bone Marrow Cells Cell Separation Concanavalin A / pharmacology Dogs Epitopes / immunology* Fluorescent Antibody Technique Histocytochemistry Lymph Nodes / cytology Lymphocyte Culture Test, Mixed Mitosis Phytohemagglutinins / pharmacology Rosette Formation T-Lymphocytes / cytology, immunology* Thymus Gland / cytology |
| Chemical | |
Reg. No./Substance:
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0/Antilymphocyte Serum; 0/Epitopes; 0/Phytohemagglutinins; 11028-71-0/Concanavalin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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