| Serological evidence for antigenic relationships between Ehrlichia canis and Cowdria ruminantium. | |
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MedLine Citation:
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PMID: 8191006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sera from dogs experimentally infected with Ehrlichia canis reacted at very similar titres in indirect fluorescent antibody (IFA) tests with E canis and Cowdria ruminantium antigen. In Western blots these sera reacted strongly with the immunodominant antigens of E canis (27 kDa) and C ruminantium (25 and 32 kDa). The dogs experimentally infected with C ruminantium showed no clinical evidence of infection, though the presence of organisms in the blood was demonstrable, for up to two weeks after inoculation, by a polymerase chain reaction. The sera from these dogs were also reactive with both organisms in the IFA tests, but the antibody titres were four- to fivefold higher with C ruminantium than with E canis antigen. In Western blots, these sera reacted strongly with the 25 and 32 kDa antigens of C ruminantium but weakly with the 27 kDa antigen of E canis. These results provide strong evidence that the immunodominant antigens of E canis and C ruminantium contain cross-reacting epitopes. |
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Authors:
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P J Kelly; L A Matthewman; S M Mahan; S Semu; T Peter; P R Mason; P Brouqui; D Raoult |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Research in veterinary science Volume: 56 ISSN: 0034-5288 ISO Abbreviation: Res. Vet. Sci. Publication Date: 1994 Mar |
Date Detail:
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Created Date: 1994-06-23 Completed Date: 1994-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0401300 Medline TA: Res Vet Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 170-4 Citation Subset: IM |
Affiliation:
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Faculty of Veterinary Science, University of Zimbabwe, Mount Pleasant, Harare. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / immunology, isolation & purification Base Sequence Blotting, Western / veterinary Cross Reactions Dog Diseases / immunology* Dogs Ehrlichia / immunology* Ehrlichia ruminantium / immunology* Ehrlichiosis / immunology, veterinary* Fluorescent Antibody Technique / veterinary Heartwater Disease / immunology* Molecular Sequence Data |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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