| Serological detection of homologies of H1o with H5 and H1 histones. | |
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MedLine Citation:
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PMID: 7024268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunospecifically purified anti-chicken H5 antibodies caused bright staining of mouse liver nuclei in an indirect immunofluorescence assay. Analysis with the fluorescence-activated cell sorter showed that 95% of the nuclei were in the brightest category. Histones extracted from mouse liver nuclei, analyzed by sodium dodecyl sulfate-gel electrophoresis, presented an extra band with the same mobility as chick erythrocyte H5. Electrophoretic blots on nitrocellulose paper were treated with anti-H5 or anti-H1 antibodies and iodinated protein A. The affinity purified anti-H5 bound to chicken H5 and mouse H1o fractions only. Anti-calf H1 bound to calf, chicken, and mouse H1 proteins and to mouse H1o as well. Antibodies to purified subfractions of rat thymus H1 showed binding to both H1 and H1o. The results support the suggestions that H1o bears significant structural homology with H5 and H1. |
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Authors:
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C V Mura; B D Stollar |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 256 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1981 Oct |
Date Detail:
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Created Date: 1981-11-22 Completed Date: 1981-11-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9767-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen-Antibody Complex Cell Nucleus / analysis* Chickens Cross Reactions Erythrocytes / analysis Fluorescent Antibody Technique Histones / analysis*, immunology Immune Sera Liver / analysis* Mice Radioimmunoassay |
| Chemical | |
Reg. No./Substance:
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0/Antigen-Antibody Complex; 0/Histones; 0/Immune Sera |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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