Document Detail

Serologic identification of hepatitis E virus infections in epidemic and endemic settings.
MedLine Citation:
PMID:  1578218     Owner:  NLM     Status:  MEDLINE    
Recombinant chimeric protein C2 containing the N-terminal region of trpE (37 kilodaltons [kDa]) and the C-terminal half (46.8 kDa) of the polypeptide encoded by ORF2 of the hepatitis E virus (HEV) genome was used for the construction of a Western blot diagnostic test for IgG and IgM antibodies to the virus (anti-HEV). (The C2 protein and the trpE protein devoid of C2 activity and used as a control for non-specific reactions were purified by recovery from sodium dodecyl sulfate-polyacrylamide gel electrophoresis [SDS-PAGE] and used for preparation of strips). Specificity of the test was proven with sera obtained from patients with acute hepatitis non-A, non-B, non-C (NANBNC) from outbreaks in different geographic regions of the world. IgG antibodies reactive to the recombinant C2 protein were detected in 93% of patients with acute hepatitis NANBNC and remained detectable in 89-100% of these patients 1-24 months after onset of jaundice. IgM antibodies were detected in 73% of patients within 26 days after onset of jaundice, in 50% 1-4 months after onset, in 6% 6-7 months after onset, and in no patients by 8 months after onset. When this test was used to identify sporadic hepatitis E cases in different regions of the world, such cases were found almost exclusively in areas where outbreaks of the disease had occurred and rarely in any other regions.
M O Favorov; H A Fields; M A Purdy; T L Yashina; A G Aleksandrov; M J Alter; D M Yarasheva; D W Bradley; H S Margolis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of medical virology     Volume:  36     ISSN:  0146-6615     ISO Abbreviation:  J. Med. Virol.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-06-10     Completed Date:  1992-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  246-50     Citation Subset:  IM    
Clinical and Diagnostic Department, D.I. Ivanofsky Institute (World Health Organization Collaborating Centre for Research and Reference on Viral Hepatitis), Academy of Medical Sciences, Moscow, USSR.
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MeSH Terms
Blotting, Western
Child, Preschool
Disease Outbreaks
Hepatitis Antibodies / blood
Hepatitis E / epidemiology*
Hepatitis E virus / classification,  immunology*
Immunoglobulin G / blood
Immunoglobulin M / blood
Middle Aged
Recombinant Proteins / immunology
Seroepidemiologic Studies
Reg. No./Substance:
0/Hepatitis Antibodies; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Recombinant Proteins

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