Document Detail


Sero-diagnosis of surra exploiting recombinant VSG antigen based ELISA for surveillance.
MedLine Citation:
PMID:  25269987     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Trypanosoma evansi, a haemoflagellate, causes "surra" an important chronic wasting disease of a wide range of wild and domestic herbivorous and carnivorous animals including cattle, buffaloes, camels, horses, etc. The untreated recovered animal can act as a carrier without exhibiting the disease symptoms and can be a source of infection to healthy animals. The diagnosis and subsequent treatment of the carrier animals is helpful to curb the disease. As the parasitaemia in carrier animals is very scanty, the conventional blood smear examination, which is widely practiced in the field, cannot detect such condition. For this purpose improved diagnostics are very much useful for mass sero-screening test such as ELISA. In the present study, the VSG of T. evansi was expressed in prokaryotic system (E. coli) and thereafter its immunoreactivity has been evaluated in immuno blot and enzyme immuno assay. The expressed protein showed 95.6% sensitivity, 98.0% specificity and 0.93 Cohen's kappa value, when compared with standard antigens. The developed antigen has also been validated with field serum samples from bovine, camel and horse collected from different states of India. The data showed that the developed recombinant antigen can be a diagnostic tool to detect carrier animals as well as control of the disease.
Authors:
P P Sengupta; G R Rudramurthy; M Ligi; M Roy; V Balamurugan; P Krishnamoorthy; M Nagalingam; L Singh; H Rahman
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-6
Journal Detail:
Title:  Veterinary parasitology     Volume:  -     ISSN:  1873-2550     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-10-1     Completed Date:  -     Revised Date:  2014-10-2    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
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