| Serine threonine receptor-associated protein (STRAP) plays a role in the maintenance of mesenchymal morphology. | |
| | |
MedLine Citation:
|
PMID: 19781628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The stromal tissue, made of extracellular matrix and mesenchymal cells, is vital for the functional design of all complex tissues. Fibroblasts are key components of stromal tissue and play a crucial role during organ development, wound repair, angiogenesis and fibrosis. We have previously reported the identification of a novel WD-domain protein, STRAP(1) that inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and TGF-beta-independent mechanisms. Here, we report, for the first time, that deletion of STRAP from Mouse Embryonic Fibroblasts (MEFs) results in a loss of mesenchymal morphology. These cells lose their spindle shape and exhibit features of an epithelial morphology. Gene expression profiling has confirmed that deletion of STRAP affects expression of sets of genes important for diverse functions including cell-cell adhesion and cell polarization, and upregulates E-cadherin expression leading to the formation of adherens junctions, subsequent localization of beta-catenin to the cell membrane and downregulation of the mesenchymal markers like LEF1 (lymphoid enhancer-binding factor 1). Upregulation of WT1 (Wilms tumor homolog 1) in STRAP null MEFs plays a role in E-cadherin induction. Finally, stable expression of STRAP in these cells results in a loss of WT1 and E-cadherin expressions, and a reversal from epithelial to the mesenchymal morphology. Thus, these results provide a novel TGF-beta-independent function of STRAP and describe a mechanism for the role of STRAP in the maintenance of mesenchymal morphology. |
| | |
Authors:
|
Nilesh D Kashikar; Jennifer Reiner; Arunima Datta; Pran K Datta |
Related Documents
:
|
22575448 - Compound k, a novel ginsenoside metabolite, inhibits adipocyte differentiation in 3t3-l... 18773488 - Integrin-linked kinase regulates e-cadherin expression through parp-1. 1988168 - The differential expression of protein kinase c genes in normal human neonatal melanocy... 12942578 - Expression of translation initiation factor eif-2alpha is increased in benign and malig... 11095928 - Tumour necrosis factor-alpha regulation of prostaglandin h synthase-2 transcription is ... 10226008 - Transgenic rescue of congenital heart disease and spina bifida in splotch mice. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-09-23 |
Journal Detail:
|
Title: Cellular signalling Volume: 22 ISSN: 1873-3913 ISO Abbreviation: Cell. Signal. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2009-10-23 Completed Date: 2009-12-14 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 8904683 Medline TA: Cell Signal Country: England |
Other Details:
|
Languages: eng Pagination: 138-49 Citation Subset: IM |
Affiliation:
|
Department of Surgery and Cancer Biology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cadherins / metabolism Cell Shape* Cells, Cultured Fibroblasts / metabolism Mesenchymal Stem Cells / cytology*, metabolism* Mice Microscopy, Electron Proteins / genetics, metabolism* Signal Transduction Transcription, Genetic Up-Regulation WT1 Proteins / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
5P50CA90949/CA/NCI NIH HHS; CA113519/CA/NCI NIH HHS; P50 CA090949-06A10005/CA/NCI NIH HHS; P50 CA090949-070005/CA/NCI NIH HHS; P50 CA090949-080005/CA/NCI NIH HHS; R01 CA095195-06A1/CA/NCI NIH HHS; R01 CA113519-01A1/CA/NCI NIH HHS; R01 CA113519-02/CA/NCI NIH HHS; R01 CA113519-03/CA/NCI NIH HHS; R01 CA113519-04/CA/NCI NIH HHS; R01 CA95195/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cadherins; 0/Proteins; 0/STRAP protein, mouse; 0/WT1 Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Loop mediated isothermal amplification combined with nucleic acid lateral flow strip for diagnosis o...
Next Document: The cardioprotective effects of zileuton, a 5-lipoxygenase inhibitor, are mediated by COX-2 via acti...