| Serine 714 might be implicated in the regulation of the phosphorylation in other areas of mPer1 protein. | |
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MedLine Citation:
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PMID: 16750171 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The phosphorylation of mPer proteins may play important roles in the mechanism of the circadian clock via changes in subcellular localization and degradation. However, the mechanism has remained unclear. Previously, we identified three putative casein kinase (CK)1epsilon phosphorylation motif clusters in mPer1. In this work, we examined the role of the phosphorylation of serine residue, Ser(S)714, in mPer1. mPer1 S[714-726]A mutant, in which potential phosphorylation serine residues replaced by alanine residues, is rapidly phosphorylated compared with wild-type mPer1 by CK1epsilon. Coexpression with S[714]G mutant of mPer1 advanced phase of circadian expression of mPer2-luc expression, which was monitored by in vitro bioluminescence system. This result showed that the mPER1 S[714]G mutation affects circadian core oscillator. Considering these, it seems that Ser 714 might be involved in the regulation of the phosphorylation of other sites in mPer1 by CK1epsilon. |
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Authors:
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Atsuko Takano; Katsuya Nagai |
Publication Detail:
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Type: Journal Article Date: 2006-05-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 346 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-19 Completed Date: 2006-08-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 95-101 Citation Subset: IM |
Affiliation:
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Institute for Protein Research, Osaka University, 3-2 Yamada-Oka, Suita, Osaka 565-0871, Japan. takano@obi.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acid Substitution Animals Binding Sites COS Cells Casein Kinase Iepsilon / metabolism Cell Cycle Proteins Cercopithecus aethiops Mice Nuclear Proteins / genetics, metabolism* Period Circadian Proteins Phosphorylation / drug effects Serine / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Nuclear Proteins; 0/Per1 protein, mouse; 0/Period Circadian Proteins; 56-45-1/Serine; EC 2.7.11.1/Casein Kinase Iepsilon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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