Document Detail

Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held.
MedLine Citation:
PMID:  17401332     Owner:  NLM     Status:  MEDLINE    
Serial section electron microscopy is typically applied to investigation of small tissue volumes encompassing subcellular structures. However, in neurobiology, the need to relate subcellular structure to organization of neural circuits can require investigation of large tissue volumes at ultrastructural resolution. Analysis of ultrastructure and three-dimensional reconstruction of even one to a few cells is time consuming, and still does not generate the necessary numbers of observations to form well-grounded insights into biological principles. We describe an assemblage of existing computer-based methods and strategies for graphical analysis of large photographic montages to accomplish the study of multiple neurons through large tissue volumes. Sample preparation, data collection and subsequent analyses can be completed within 3-4 months. These methods generate extremely large data sets that can be mined in future studies of nervous system organization.
Brian K Hoffpauir; Brian A Pope; George A Spirou
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Nature protocols     Volume:  2     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-06-29     Revised Date:  2008-03-24    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  9-22     Citation Subset:  IM    
Department of Otolaryngology, PO Box 9303, Health Sciences Center, One Medical Center Drive, West Virginia University School of Medicine, Morgantown, West Virginia 26506-9303, USA.
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MeSH Terms
Anatomy, Cross-Sectional / methods*
Image Processing, Computer-Assisted / methods*
Microscopy, Electron / methods*
Neurons / ultrastructure*
Grant Support

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