Document Detail


Serial analysis of gene expression in HIV-1-infected T cell lines.
MedLine Citation:
PMID:  10580116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gene expression profile of the HIV-1 infection state was analyzed in the human T cell line MOLT-4. Using the serial analysis of gene expression (SAGE) method, a total of 142¿ omitted¿603 SAGE tags were sequenced and identified, representing 43¿ omitted¿581 unique mRNA species. Comparison of expression patterns revealed that 53 cellular genes were differentially expressed upon HIV-1 infection. Northern blot and RT-PCR analyses confirmed the altered expression of the genes in both MOLT-4 and MT-4 cells. Up-regulated genes were mainly composed of transcription factors and genes related to T cell activation, whereas down-regulated genes were comprised of mitochondrial proteins, actin-related factors and translational factors. These findings indicate that persistent T cell activation, which may accelerate HIV-1 replication, and the disruption of cellular housekeeping genes including those involved in anti-apoptotic systems, may play an important role in HIV-1-induced pathogenesis.
Authors:
A Ryo; Y Suzuki; K Ichiyama; T Wakatsuki; N Kondoh; A Hada; M Yamamoto; N Yamamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  462     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-01-13     Completed Date:  2000-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  182-6     Citation Subset:  IM; X    
Affiliation:
Departments of Microbiology and Molecular Virology, School of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics
Expressed Sequence Tags
Gene Expression Profiling*
HIV Infections
HIV-1*
Humans
Inhibitor of Apoptosis Proteins
Lymphocyte Activation
Proteins / genetics
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / metabolism,  virology*
Tumor Cells, Cultured
Virus Replication
Chemical
Reg. No./Substance:
0/Inhibitor of Apoptosis Proteins; 0/Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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