| Serial Analysis of Gene Expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation. | |
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MedLine Citation:
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PMID: 12955499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Treatment with cyclic AMP (cAMP) induces appressorium formation in the phytopathogenic fungus Magnaporthe grisea, the causative agent of rice blast disease. In a search for the M. grisea genes responsible for appressorium formation and host invasion, SAGE (Serial Analysis of Gene Expression) was carried out using mRNA isolated from fungal conidia germinating in the presence and absence of cAMP. From cAMP-treated conidia 5087 tags including 2889 unique tags were isolated, whereas untreated conidia yielded 2342 unique tags out of total of 3938. cAMP treatment resulted in up- and down-regulation of genes corresponding to 57 and 53 unique tags, respectively. Upon consultation of EST/cDNA databases, 22 tags with higher representation in cAMP-treated conidia were annotated with putative gene names. Furthermore, 28 tags corresponding to cAMP-induced genes could be annotated with the help of the recently published genome sequence of M. grisea. cAMP-induced genes identified by SAGE included many genes that have not been described so far, as well as a number of genes known to be involved in pathogenicity, e.g. MPG1, MAS1 and MAC1. RT-PCR of 13 randomly selected genes confirmed the SAGE results, verifying the fidelity of the SAGE data. |
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Authors:
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T Irie; H Matsumura; R Terauchi; H Saitoh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-08-29 |
Journal Detail:
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Title: Molecular genetics and genomics : MGG Volume: 270 ISSN: 1617-4615 ISO Abbreviation: Mol. Genet. Genomics Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-11-07 Completed Date: 2004-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101093320 Medline TA: Mol Genet Genomics Country: Germany |
Other Details:
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Languages: eng Pagination: 181-9 Citation Subset: IM |
Affiliation:
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Department of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cyclic AMP / pharmacology DNA, Fungal / genetics Gene Expression Profiling Gene Expression Regulation, Fungal / drug effects Genes, Fungal* / drug effects Magnaporthe / drug effects, genetics*, growth & development, pathogenicity Oryza sativa / microbiology Plant Diseases / microbiology Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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