Document Detail

Sequestration of retinyl esters is essential for retinoid signaling in the zebrafish embryo.
MedLine Citation:
PMID:  17098734     Owner:  NLM     Status:  MEDLINE    
For vertebrate development, vitamin A (all-trans retinol) is required in quantitative different amounts and spatiotemporal distribution for the production of retinoic acid, a nuclear hormone receptor ligand, and 11-cis retinal, the chromophore of visual pigments. We show here for zebrafish that embryonic retinoid homeostasis essentially depends on the activity of a leci-thin:retinol acyltransferase (Lratb). During embryogenesis, lratb is expressed in mostly non-overlapping domains opposite to retinal dehydrogenase 2 (raldh2), the key enzyme for retinoic acid synthesis. Blocking retinyl ester formation by a targeted knock down of Lratb results in significantly increased retinoic acid levels, which lead to severe embryonic patterning defects. Thus, we provide evidence that a balanced competition between Lratb and Raldh2 for yolk vitamin A defines embryonic compartments either for retinyl ester or retinoic acid synthesis. This homeostatic mechanism dynamically adjusts embryonic retinoic acid levels for gene regulation, concomitantly sequestering excess yolk vitamin A in the form of retinyl esters for the establishment of larval vision later during development.
Andrea Isken; Jochen Holzschuh; Johanna M Lampert; Lara Fischer; Vitus Oberhauser; Krzysztof Palczewski; Johannes von Lintig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-10
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  282     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-08     Completed Date:  2007-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1144-51     Citation Subset:  IM    
Department of Neurobiology, Institute of Biology I, Albert-Ludwigs University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany.
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MeSH Terms
Acyltransferases / genetics,  metabolism
Aldehyde Oxidoreductases / genetics,  metabolism
Amino Acid Sequence
Cell Line
Egg Yolk / metabolism
Embryo, Nonmammalian / embryology,  metabolism
Esters / metabolism
Eye / embryology*,  metabolism*
Gene Expression Regulation, Developmental
Homeostasis / physiology
Molecular Sequence Data
Retinaldehyde / metabolism
Retinoids / metabolism*
Signal Transduction / physiology*
Tretinoin / metabolism
Vitamin A / metabolism
Zebrafish / embryology*,  metabolism*
Reg. No./Substance:
0/Esters; 0/Retinoids; 11103-57-4/Vitamin A; 116-31-4/Retinaldehyde; 302-79-4/Tretinoin; EC 1.2.-/Aldehyde Oxidoreductases; EC 2.3.-/Acyltransferases; EC 2.3.1.-/lecithin-retinol acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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