| Sequestration of retinyl esters is essential for retinoid signaling in the zebrafish embryo. | |
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MedLine Citation:
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PMID: 17098734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For vertebrate development, vitamin A (all-trans retinol) is required in quantitative different amounts and spatiotemporal distribution for the production of retinoic acid, a nuclear hormone receptor ligand, and 11-cis retinal, the chromophore of visual pigments. We show here for zebrafish that embryonic retinoid homeostasis essentially depends on the activity of a leci-thin:retinol acyltransferase (Lratb). During embryogenesis, lratb is expressed in mostly non-overlapping domains opposite to retinal dehydrogenase 2 (raldh2), the key enzyme for retinoic acid synthesis. Blocking retinyl ester formation by a targeted knock down of Lratb results in significantly increased retinoic acid levels, which lead to severe embryonic patterning defects. Thus, we provide evidence that a balanced competition between Lratb and Raldh2 for yolk vitamin A defines embryonic compartments either for retinyl ester or retinoic acid synthesis. This homeostatic mechanism dynamically adjusts embryonic retinoic acid levels for gene regulation, concomitantly sequestering excess yolk vitamin A in the form of retinyl esters for the establishment of larval vision later during development. |
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Authors:
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Andrea Isken; Jochen Holzschuh; Johanna M Lampert; Lara Fischer; Vitus Oberhauser; Krzysztof Palczewski; Johannes von Lintig |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-10 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2007-01-08 Completed Date: 2007-03-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1144-51 Citation Subset: IM |
Affiliation:
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Department of Neurobiology, Institute of Biology I, Albert-Ludwigs University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acyltransferases
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genetics,
metabolism Aldehyde Oxidoreductases / genetics, metabolism Amino Acid Sequence Animals Cell Line Drosophila Egg Yolk / metabolism Embryo, Nonmammalian / embryology, metabolism Esters / metabolism Eye / embryology*, metabolism* Gene Expression Regulation, Developmental Homeostasis / physiology Molecular Sequence Data Retinaldehyde / metabolism Retinoids / metabolism* Signal Transduction / physiology* Tretinoin / metabolism Vitamin A / metabolism Zebrafish / embryology*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Retinoids; 11103-57-4/Vitamin A; 116-31-4/Retinaldehyde; 302-79-4/Tretinoin; EC 1.2.-/Aldehyde Oxidoreductases; EC 2.3.-/Acyltransferases; EC 2.3.1.-/lecithin-retinol acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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