Document Detail


Sequential staining of DNA-aldehyde with Schiff's reagent and acriflavine-SO2.
MedLine Citation:
PMID:  81160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acid hydrolysed DNA of rat liver was stained with Schiff's reagent at pHs 1.7 or 3.0 followed by staining with acriflavine-SO2 at pH 2.0 as well as with acriflavine-SO2 followed by Schiff's reagent at pH 1.7 or 3.0. Nuclei stained with Schiff's reagent at pH 1.7 were brown-yellow and an analysis of their absorption characteristics revealed two peaks--one at 470 nm and the other at 570 nm. Although nuclei stained with Schiff's reagent at pH 3.0 followed by acriflavine-SO2 were deep magenta in colour, they also showed similar peaks of maximum absorption. Identical peaks were also seen when the sequence of staining was reversed. It is suggested that in the conventional Feulgen-type reactions only some of the DNA-aldehyde molecules are stained; the remaining molecules can be stained by sequential application of another Schiff or Schiff-type reagent such as acriflavine-SO2. The possible mechanism of staining in these cases has been discussed.
Authors:
M K Dutt
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Folia histochemica et cytochemica     Volume:  16     ISSN:  0015-5586     ISO Abbreviation:  Folia Histochem Cytochem (Krakow)     Publication Date:  1978  
Date Detail:
Created Date:  1978-12-20     Completed Date:  1978-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984760R     Medline TA:  Folia Histochem Cytochem (Krakow)     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  241-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acridines / diagnostic use*
Acriflavine / diagnostic use*
Aldehydes / analysis
Animals
DNA / analysis*
Hydrogen-Ion Concentration
Liver / analysis*
Rats
Rosaniline Dyes / diagnostic use
Staining and Labeling / methods*
Sulfhydryl Compounds / diagnostic use*
Chemical
Reg. No./Substance:
0/Acridines; 0/Aldehydes; 0/Rosaniline Dyes; 0/Sulfhydryl Compounds; 8048-52-0/Acriflavine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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