Document Detail


Sequential silver staining and hybridization in situ on nucleolus organizing regions in human cells.
MedLine Citation:
PMID:  89936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome preparations from eight individuals were first stained with silver nitrate to reveal the nucleolus organizing regions (NORs) and then hybridized in situ with ribosomal RNA. In six individuals the size of the silver-staining regions was positively correlated with the amount of label present after hybridization in situ. Thus the variation in silver-staining intensity among chromosomes was largely explained by variation in the number of rDNA gene copies per NOR. However, in two individuals this correlation was absent, suggesting that other factors can also influence the size of the silver-staining region.
Authors:
D Warburton; A S Henderson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  24     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1979  
Date Detail:
Created Date:  1979-11-29     Completed Date:  1979-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  168-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosome Banding
Humans
Hybrid Cells / metabolism,  ultrastructure
Metaphase
Nucleic Acid Hybridization*
Nucleolus Organizer Region / ultrastructure*
RNA, Ribosomal / metabolism*
Silver / diagnostic use*
Staining and Labeling
Chemical
Reg. No./Substance:
0/RNA, Ribosomal; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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