Document Detail


Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices.
MedLine Citation:
PMID:  16429794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples.
Authors:
Jaroon Jakmunee; Thitima Rujiralai; Kate Grudpan
Related Documents :
7887464 - Assay of ornithine aminotransferase with ninhydrin.
18423474 - Use of supercritical fluid extraction and gas chromatography-mass spectrometry to obtai...
20298854 - Flow-injection catalytic spectrophotometic determination of molybdenum(vi) in plants us...
10527524 - A fluorescence-based high-performance liquid chromatographic assay to determine acid ce...
6626624 - Plasma amino acid ratio as an index of hepatocellular maturity in the neonate.
1683804 - Use of high performance liquid chromatography in defining the abnormalities in the free...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  22     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-24     Completed Date:  2006-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  157-60     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. scijjkmn@chiangmai.ac.th
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Beverages / analysis*
Calibration
Citric Acid / analysis*
Flow Injection Analysis / instrumentation,  methods
Food Analysis / instrumentation,  methods
Fruit / chemistry*
Hydrogen-Ion Concentration
Indicators and Reagents
Phenolphthalein / chemistry
Sensitivity and Specificity
Sodium Hydroxide / chemistry
Spectrophotometry / instrumentation,  methods
Time Factors
Titrimetry / instrumentation,  methods
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 1310-73-2/Sodium Hydroxide; 77-09-8/Phenolphthalein; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Simple spectrophotometric flow injection system with an in-valve minicolumn for enhancement during t...
Next Document:  Speciation of chromium in seawater by ICP-AES with dual mini-columns containing chelating resin.