Document Detail

Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices.
MedLine Citation:
PMID:  16429794     Owner:  NLM     Status:  MEDLINE    
A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples.
Jaroon Jakmunee; Thitima Rujiralai; Kate Grudpan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  22     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-24     Completed Date:  2006-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  157-60     Citation Subset:  IM    
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
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MeSH Terms
Beverages / analysis*
Citric Acid / analysis*
Flow Injection Analysis / instrumentation,  methods
Food Analysis / instrumentation,  methods
Fruit / chemistry*
Hydrogen-Ion Concentration
Indicators and Reagents
Phenolphthalein / chemistry
Sensitivity and Specificity
Sodium Hydroxide / chemistry
Spectrophotometry / instrumentation,  methods
Time Factors
Titrimetry / instrumentation,  methods
Reg. No./Substance:
0/Indicators and Reagents; 1310-73-2/Sodium Hydroxide; 77-09-8/Phenolphthalein; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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