Document Detail

Sequential induction of marrow stromal cells by FGF2 and BMP2 improves their growth and differentiation potential in vivo.
MedLine Citation:
PMID:  21030007     Owner:  NLM     Status:  In-Process    
BACKGROUND: repairing bone loss by autologous grafting requires that a patient's marrow stromal cells (MSCs) be collected and cultured until the number of cells is adequate for implantation. Currently used techniques allow a slow proliferation rate and produce a culture that contains only small amounts of pluripotent stem cells that will become osteoblasts in culture.
OBJECTIVE: to develop culture conditions that permit a rapid increase in the number of MSCs while retaining or improving their potential for complete differentiation in vivo.
RESULTS: sequential applications of low doses of basic fibroblast growth factor 2 (FGF2) and bone morphogenetic protein 2 (BMP2) improved the growth and differentiation potential of MSCs. FGF2 also elevated sensitivity of the cells to BMP2. BMP2 increased the syntheses of alkaline phosphatase (ALP), collagen type I and bone sialoprotein, while FGF2 increased the expression of osteocalcin (OC). Full induction as determined by the formation of mineralised nodules in vitro was observed within 7 days. Seeding the induced cells onto scaffolds and then implanting them into nude mice resulted in newly formed bone 4 weeks later. The results of real-time polymerase chain reaction (PCR) and Western blotting suggested that FGF2 increased the pool of committed osteoblasts by up-regulating the Cbfa1/Runx2 gene. The later stages of bone formation seemed to be induced by Cbfa1/Runx2-downstream factors such as BMP2, ALP, collagen type I, bone sialoprotein and OC.
CONCLUSION: the culture system that was developed increased both the proliferation of MSC and the proportion that developed into pre-osteoblasts.
J Kaewsrichan; P Wongwitwichot; K Chandarajoti; K H Chua; B H I Ruszymah
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-27
Journal Detail:
Title:  Archives of oral biology     Volume:  56     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  90-101     Citation Subset:  D; IM    
Copyright Information:
2010 Elsevier Ltd. All rights reserved.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Kanjanavanish Rd., Hat-Yai, Songkhla 90112, Thailand.
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