| Sequential induction of Hsp25 and proliferating cell nuclear antigen in the kidney after burn. | |
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MedLine Citation:
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PMID: 15215048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Burn injury elicits a wide range of intracellular signaling events leading to alterations in phenotypes of distant organs. Renal dysfunction is one of several serious postburn complications. To better understand the underlying mechanisms of renal dysfunction among burn patients, we investigated alterations in the expression of heat shock proteins (Hsps) and cell cycle-associated proteins in the kidney after burn. Following an approximately 18% total body surface area burn, blood and kidney samples were harvested from mice at several time points. Serum levels of blood urea nitrogen increased significantly at 3 h and returned to basal levels at Day 1 implying a transient dysfunction of glomerular filtration. The expression of Hsp25 was increased at Day 1, whereas no changes in Hsp70 expression were observed. An increase in proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, peaked at Day 3, and its expression was predominantly limited to cells appearing to be tubular epithelial cells in the cortex. In contrast, no significant alterations in the p21 mitosis inhibitor were noted. Furthermore, increases in histones H1 and H2A at Day 3 paralleled the PCNA induction suggesting a burn-mediated alteration in cell cycle activities. The results from this study suggest that a sizeable burn may trigger sequential activation of signaling events involved in the early pathogenesis and subsequent recovery of the kidney after burn. |
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Authors:
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Jayoung Jeong; David G Greenhalgh; Kiho Cho |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental and molecular pathology Volume: 77 ISSN: 0014-4800 ISO Abbreviation: Exp. Mol. Pathol. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-06-24 Completed Date: 2004-08-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370711 Medline TA: Exp Mol Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 35-42 Citation Subset: IM |
Affiliation:
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Burn Research, Shriners Hospitals for Children Northern California and Department of Surgery, University of California at Davis, Sacramento, CA 95817, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Urea Nitrogen Blotting, Western Burns / blood, metabolism*, pathology Cyclin-Dependent Kinase Inhibitor p21 Cyclins / biosynthesis, genetics Disease Models, Animal Female Gene Expression Regulation HSP70 Heat-Shock Proteins / biosynthesis Heat-Shock Proteins / biosynthesis*, genetics Histones / metabolism Immunoenzyme Techniques Kidney / metabolism*, pathology Mice Mice, Inbred C57BL Neoplasm Proteins / biosynthesis*, genetics Proliferating Cell Nuclear Antigen / biosynthesis* RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Histones; 0/Hspb1 protein, mouse; 0/Neoplasm Proteins; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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