Document Detail

Sequential induction of Hsp25 and proliferating cell nuclear antigen in the kidney after burn.
MedLine Citation:
PMID:  15215048     Owner:  NLM     Status:  MEDLINE    
Burn injury elicits a wide range of intracellular signaling events leading to alterations in phenotypes of distant organs. Renal dysfunction is one of several serious postburn complications. To better understand the underlying mechanisms of renal dysfunction among burn patients, we investigated alterations in the expression of heat shock proteins (Hsps) and cell cycle-associated proteins in the kidney after burn. Following an approximately 18% total body surface area burn, blood and kidney samples were harvested from mice at several time points. Serum levels of blood urea nitrogen increased significantly at 3 h and returned to basal levels at Day 1 implying a transient dysfunction of glomerular filtration. The expression of Hsp25 was increased at Day 1, whereas no changes in Hsp70 expression were observed. An increase in proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, peaked at Day 3, and its expression was predominantly limited to cells appearing to be tubular epithelial cells in the cortex. In contrast, no significant alterations in the p21 mitosis inhibitor were noted. Furthermore, increases in histones H1 and H2A at Day 3 paralleled the PCNA induction suggesting a burn-mediated alteration in cell cycle activities. The results from this study suggest that a sizeable burn may trigger sequential activation of signaling events involved in the early pathogenesis and subsequent recovery of the kidney after burn.
Jayoung Jeong; David G Greenhalgh; Kiho Cho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  77     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-06-24     Completed Date:  2004-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35-42     Citation Subset:  IM    
Burn Research, Shriners Hospitals for Children Northern California and Department of Surgery, University of California at Davis, Sacramento, CA 95817, USA.
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MeSH Terms
Blood Urea Nitrogen
Blotting, Western
Burns / blood,  metabolism*,  pathology
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / biosynthesis,  genetics
Disease Models, Animal
Gene Expression Regulation
HSP70 Heat-Shock Proteins / biosynthesis
Heat-Shock Proteins / biosynthesis*,  genetics
Histones / metabolism
Immunoenzyme Techniques
Kidney / metabolism*,  pathology
Mice, Inbred C57BL
Neoplasm Proteins / biosynthesis*,  genetics
Proliferating Cell Nuclear Antigen / biosynthesis*
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Histones; 0/Hspb1 protein, mouse; 0/Neoplasm Proteins; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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