Document Detail

Sequential in-office vitreous aspirates demonstrate vitreous matrix metalloproteinase 9 levels correlate with the amount of subretinal fluid in eyes with wet age-related macular degeneration.
MedLine Citation:
PMID:  22773904     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To evaluate levels of 37 native pathway proteins of the vitreous proteome from a subset of wet age-related macular degeneration (AMD) patients with and without subretinal fluid (SRF).
METHODS: A total of 62 consecutive samples were aspirated from 12 patients with AMD, six who had SRF at baseline, and six who did not have SRF at any point during the study. Vitreous levels of the 37 native pathway proteins were analyzed in these patients using reverse phase protein microarray technology. At each visit, at which the 62 samples were taken, SRF and central retinal thickness were measured. These values were then compared to the relative intensity level of the 37 proteins screened.
RESULTS: In the subset of AMD patients with SRF, the average matrix metalloproteinase 9 (MMP-9), interleukin (IL)-12, Abelson murine leukemia viral oncogene homolog 1 (cABL) Thr735, heme oxygenase-1, Musashi, platelet-derived growth factor receptor beta Tyr751 (PDGFRβ), IL-8, and BCL-2 associated death promoter (BAD) Ser112 levels in the vitreous were found to be significantly different with a 21%-82% increase in expression compared to those without SRF (p<0.0001). Within the SRF group, there was a positive correlation between the vitreous MMP-9 levels and the SRF level. MMP-9 levels in the vitreous proteome varied with the level of SRF but not retinal edema. Compared to patients without SRF, the patients with initial SRF had persistent or progressive disease.
CONCLUSIONS: This is the first prospective case series sequentially monitoring the vitreous proteome in patients with wet AMD. The results suggest that MMP-9 is a proteomic biomarker of SRF accumulation, separate from macular edema.
Stephanie M Ecker; Scott M Pfahler; Joshua C Hines; Ann S Lovelace; Bert M Glaser
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-20
Journal Detail:
Title:  Molecular vision     Volume:  18     ISSN:  1090-0535     ISO Abbreviation:  Mol. Vis.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-09     Completed Date:  2012-11-13     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9605351     Medline TA:  Mol Vis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1658-67     Citation Subset:  IM    
The National Retina Institute, Department of Ocular Proteomics, Towson MD 21204, USA.
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MeSH Terms
Aged, 80 and over
Biological Markers / metabolism
Biopsy, Fine-Needle
Eye Proteins / genetics,  metabolism
Gene Expression*
Macular Degeneration / enzymology,  genetics*,  pathology
Matrix Metalloproteinase 9 / genetics*,  metabolism
Middle Aged
Prospective Studies
Protein Array Analysis
Proteome / genetics,  metabolism
Subretinal Fluid / secretion*
Vitreous Body / chemistry*,  pathology
Grant Support
1 R21 EY018942-01A1/EY/NEI NIH HHS; 1R 43EY02182-01/EY/NEI NIH HHS; 1R43EY021082-01/EY/NEI NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Eye Proteins; 0/Proteome; EC Metalloproteinase 9

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