Document Detail


Sequential gel mobility shift scanning of 5' upstream sequences of the Neurospora crassa am (GDH) gene.
MedLine Citation:
PMID:  8121395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used gel mobility shift assays to scan 1.7 kb of 5' non-coding sequence of the am (glutamate dehydrogenase) gene of Neurospora crassa for binding by partially fractionated Neurospora proteins. Using genetic analysis this region had been shown to play an important role in the control of glutamate dehydrogenase (GDH) expression. Gel mobility shift analysis identified three regions to which Neurospora proteins bind specifically. Two of these corresponded to the two elements previously defined by genetic analysis (URSam alpha and URSam beta). The third protein binding site appears to be unrelated to am gene expression. Competition experiments showed that the proteins that bind to the URSam alpha and URSam beta elements are different. The URSam alpha element was shown to contain two independent binding sites for the URSam alpha binding protein(s). Both fragments contain a CCAAT motif, suggesting that URSam alpha binding protein(s) may be members of one of the CCAAT-binding protein families. The effect of deletion of either the URSam alpha or URSam beta elements on catabolite induction of am expression was also determined. Both elements appear to act as constitutive enhancers of gene expression.
Authors:
H Chen; J A Kinsey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  242     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-06     Completed Date:  1994-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  399-403     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160-7420.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Gene Expression Regulation, Fungal
Genes, Fungal*
Glutamate Dehydrogenase / genetics*
Neurospora crassa / genetics*
Protein Binding
Regulatory Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
EC 1.4.1.2/Glutamate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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