| Sequential culture medium promotes the in vitro development of Macaca fascicularis embryos to blastocysts. | |
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MedLine Citation:
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PMID: 12210672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro production of blastocyst stage embryos from Macaca fascicularis (Mf) has not previously been demonstrated without cell support. Historical data indicates that a large proportion of Mf embryos arrest at the morula stage in nonsequential culture medium (NSM) lacking serum supplementation and/or cell support. Here we report the application of a sequential culture system supporting in vitro production of Mf blastocysts. Mf embryos produced by in vitro fertilization (IVF; n = 69) were subjected to in vitro culture without cell support in either a commercial sequential embryo culture medium (SM) or an NSM. At 24 hr post-insemination (PI) embryos generated from in vivo and in vitro matured oocytes and cultured in the NSM cleaved to two or more cells in significantly greater proportions (15/23; 65%) compared to embryos cultured in SM (14/46; 30%). However, by day 3 PI embryo development beyond eight cells was not different in NSM (9/23; 39%) compared to SM (25/46; 54%). At day 5 PI embryo development to the morula stage was slightly lower in NSM (8/23, 35%) compared to SM (21/46, 45%), and embryo degeneration was slightly higher in NSM (9/23, 39%) compared to SM (9/46, 20%). After 7-9 days of in vitro culture, embryo development to the blastocyst stage and embryo degeneration were significantly lower and higher, respectively, in NSM (0/23, 0%; and 23/23, 100%) compared to SM (9/46, 20%; and 26/46, 56%). In this study the sequential culture system was better able to support in vitro development of Mf embryos compared to nonsequential culture systems. |
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Authors:
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E C Curnow; D Pawitri; E S Hayes |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of primatology Volume: 57 ISSN: 0275-2565 ISO Abbreviation: Am. J. Primatol. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-09-04 Completed Date: 2002-10-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8108949 Medline TA: Am J Primatol Country: United States |
Other Details:
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Languages: eng Pagination: 203-12 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Wiley-Liss, Inc. |
Affiliation:
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Monash Institute of Reproduction and Development, Clayton, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst Culture Media Embryonic and Fetal Development Female Fertilization in Vitro / veterinary* Macaca fascicularis / embryology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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