| Sequential appearance of relaxin, prolactin and IGFBP-1 during growth and differentiation of the human endometrium. | |
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MedLine Citation:
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PMID: 7694877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Relaxin (RLX) is a product of the human corpus luteum, pregnancy decidua and placenta, prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1) are products of the cyclic endometrium and of the pregnancy decidua. All three proteins are thought to function interdependently in endometrium/decidua as local factors within the uterus without reaching the systemic circulation. In this study, the avidin-biotin immunoperoxidase method for immunolocalization with monoclonal or polyclonal antibodies has been applied to serial sections of endometria obtained from patients at different stages of the menstrual cycle and in early and late gestation. This allowed the cellular localization of the three proteins to be followed simultaneously through the reproductive stages from cyclic endometrium to term gestational decidua. The production, as opposed to sequestration of RLX from an ovarian source was demonstrated by the application in parallel of an antibody to the processed hormone and its connecting peptide. RLX was shown localized to the glandular and luminal epithelia in the proliferative and secretory phases. The decidualized stromal cells also immunostained for RLX in the late secretory phase and in early and late pregnancy. PRL was localized first to the glandular epithelium and then stroma, appearing after RLX, IGFBP-1 appeared later in the secretory phase and predominantly in the decidualized stromal cells confirming previous studies. In contrast, all three proteins were immunostained in early pregnancy and increased to term gestation.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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G D Bryant-Greenwood; E M Rutanen; S Partanen; T K Coelho; S Y Yamamoto |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 95 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-01-06 Completed Date: 1994-01-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: IRELAND |
Other Details:
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Languages: eng Pagination: 23-9 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Reproductive Biology, University of Hawaii, Honolulu 96822. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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biosynthesis* Cell Differentiation Decidua / cytology, metabolism Endometrium / cytology, growth & development, metabolism* Female Gene Expression Regulation Humans Immunoenzyme Techniques Insulin-Like Growth Factor Binding Protein 1 Menstrual Cycle Pregnancy Prolactin / biosynthesis* Relaxin / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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G12-RR-03061/RR/NCRR NIH HHS; GM07684/GM/NIGMS NIH HHS; HD-24314/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Insulin-Like Growth Factor Binding Protein 1; 9002-62-4/Prolactin; 9002-69-1/Relaxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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