| Sequencing of an internal transcribed spacer region of 16S-23S rRNA gene and designing of PCR primers for the detection of Salmonella spp. in food. | |
| | |
MedLine Citation:
|
PMID: 15582736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
DNA sequences of an internal transcribed spacer (ITS) region for 40 Salmonella serovars were determined and compared with ITS sequences of Salmonella spp., and non-Salmonella spp. already available on the GenBank database. From such comparison, two Salmonella-specific ITS based PCR primers, ITSF and ITSR, were designed. When Salmonella strains with various serotypes were PCR assayed with primers ITSF/ITSR, all generated PCR products with molecular weight bands equal to 312 bp. On the other hand, 48 non-Salmonella isolates, including strains of Enterobacteriaceae and other food pathogens generated negative results. Detection limits of this PCR method was 1-9 CFU per assay. These PCR primers were used for the detection of Salmonella cells in artificially contaminated foods, including chicken meat and whole milk. The detection limit was 1-9 x 10(3) CFU per assay. With an 8-h enrichment step performed prior to the PCR assay, however, the detection limit became 1-9 CFU per gram of the food sample. |
| | |
Authors:
|
Tasi-Hsin Chiu; Tong-Rong Chen; Wen-Zhe Hwang; Hau-Yang Tsen |
Related Documents
:
|
7549536 - Recovery of salmonella spp. from refrigerated preenrichment cultures of dry food compos... 17580626 - Precollaborative study of the genequence salmonella assay using 24-hour enrichment prot... 10641936 - Use of fecal material to associate a suspect with a crime scene: report of two cases. 8750666 - Salmonella, campylobacter and escherichia coli o157:h7 decontamination techniques for t... 1815376 - Qsars for selected aliphatic and aromatic amines. 10744896 - "pass the ketchup, please": familiar flavors increase children's willingness to taste n... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of food microbiology Volume: 97 ISSN: 0168-1605 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2005 Jan |
Date Detail:
|
Created Date: 2004-12-07 Completed Date: 2005-02-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 259-65 Citation Subset: IM |
Affiliation:
|
Department of Food Science, National Chung-Hsing University, Taichung, Taiwan, ROC. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chickens Colony Count, Microbial DNA Primers DNA, Bacterial / analysis* DNA, Ribosomal / analysis* Food Contamination / analysis Food Microbiology Humans Meat / microbiology Milk / microbiology Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 23S / genetics Salmonella / genetics*, isolation & purification* Sensitivity and Specificity Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 23S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of an electronic tongue and HPLC with electrochemical detection to differentiate molds in cultur...
Next Document: Enterococcus species dominating in fresh modified-atmosphere-packaged, marinated broiler legs are ov...