Document Detail

Sequencing of an internal transcribed spacer region of 16S-23S rRNA gene and designing of PCR primers for the detection of Salmonella spp. in food.
MedLine Citation:
PMID:  15582736     Owner:  NLM     Status:  MEDLINE    
DNA sequences of an internal transcribed spacer (ITS) region for 40 Salmonella serovars were determined and compared with ITS sequences of Salmonella spp., and non-Salmonella spp. already available on the GenBank database. From such comparison, two Salmonella-specific ITS based PCR primers, ITSF and ITSR, were designed. When Salmonella strains with various serotypes were PCR assayed with primers ITSF/ITSR, all generated PCR products with molecular weight bands equal to 312 bp. On the other hand, 48 non-Salmonella isolates, including strains of Enterobacteriaceae and other food pathogens generated negative results. Detection limits of this PCR method was 1-9 CFU per assay. These PCR primers were used for the detection of Salmonella cells in artificially contaminated foods, including chicken meat and whole milk. The detection limit was 1-9 x 10(3) CFU per assay. With an 8-h enrichment step performed prior to the PCR assay, however, the detection limit became 1-9 CFU per gram of the food sample.
Tasi-Hsin Chiu; Tong-Rong Chen; Wen-Zhe Hwang; Hau-Yang Tsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  97     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  259-65     Citation Subset:  IM    
Department of Food Science, National Chung-Hsing University, Taichung, Taiwan, ROC.
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MeSH Terms
Colony Count, Microbial
DNA Primers
DNA, Bacterial / analysis*
DNA, Ribosomal / analysis*
Food Contamination / analysis
Food Microbiology
Meat / microbiology
Milk / microbiology
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
RNA, Ribosomal, 23S / genetics
Salmonella / genetics*,  isolation & purification*
Sensitivity and Specificity
Species Specificity
Reg. No./Substance:
0/DNA Primers; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; 0/RNA, Ribosomal, 23S

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