Document Detail


Sequence variations of hepatitis B virus promoter regions in persistently infected patients.
MedLine Citation:
PMID:  11315638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HBV in the sera of two chronic active hepatitis patients were analyzed for the promoter sequence heterogeneity. In most cases, the proportion of any particular clone in the total viral populations was less than 50%, showing high mutation rates. In contrast, promoter sequences of HBV from asymptomatic carriers revealed only a few point mutations with no deletions. HBV in chronic patient harbored variants with multiple mutations throughout promoters including 1762 (A-to-T), 1764 (G-to-A) double mutation in C promoter and deletions near CCAAT site in S promoter. Unlike other three promoter regions, C, pre-S1 and S, of HBV which revealed a high level of sequence heterogeneity, the X promoter region (from nt985 to 1430) showed little sequence heterogeneity within a patient. However, the predominant viral clones in two patients were quite different from each other. In addition to mutations in promoter regions, a deletion mutation in the translation start codon was also found in pre-S1 gene. The results in this report indicate that the mutation rates are not the same in all four promoters and that one of the strategies for maintaining persistent infection could be through mutations in viral promoters which then impair the balance of viral gene expressions.
Authors:
Y M Ha-Lee; J Lee; H Pyun; Y Kim; J Sohn; Y J Cho; Y Kim
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  146     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  2001  
Date Detail:
Created Date:  2001-04-23     Completed Date:  2001-05-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  279-92     Citation Subset:  IM    
Affiliation:
Korea Nutrition Research Institute, Korea University, Seoul, Korea.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF276519;  AF276520;  AF276521;  AF276522;  AF276523;  AF276524;  AF276525;  AF276526;  AF276527;  AF276528;  AF276529;  AF276530;  AF276531;  AF276532;  AF276533;  AF276534;  AF276535;  AF276536;  AF276537;  AF276538;  AF276539;  AF276540;  AF276541;  AF276542;  AF276543;  AF276544;  AF276545;  AF276546;  AF276547;  AF276548;  AF276549;  AF276550;  AF276551;  AF276552;  AF276553;  AF276554;  AF276555;  AF276556;  AF276557;  AF276558;  AF276559;  AF276560;  AF276561;  AF276562;  AF276563;  AF276564;  AF276565;  AF276566;  AF276567;  AF276568;  AF276569;  AF276570;  AF276571;  AF276572;  AF276573;  AF276574;  AF276575;  AF276576;  AF276577;  AF276578;  AF276579;  AF276580;  AF276581;  AF276582;  AF276583;  AF276584;  AF276585;  AF276586;  AF276587;  AF276588;  AF276589;  AF276590;  AF276591;  AF276592;  AF276593;  AF276594;  AF276595;  AF276596;  AF276597;  AF276598;  AF276599;  AF276600;  AF276601;  AF276602;  AF276603;  AF276604;  AF276605;  AF276606;  AF276607;  AF276608;  AF276609;  AF276610;  AF276611;  AF276612;  AF276613;  AF276614;  AF276615;  AF276616
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MeSH Terms
Descriptor/Qualifier:
Adult
Base Sequence
Cloning, Molecular
DNA, Viral / genetics
Female
Genetic Variation*
Hepatitis B virus / genetics*,  isolation & purification
Hepatitis B, Chronic / blood,  virology*
Humans
Male
Middle Aged
Molecular Sequence Data
Mutation*
Mutation, Missense
Polymerase Chain Reaction
Promoter Regions, Genetic*
Sequence Deletion
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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