| Sequence-specific nucleic acid detection from binary pore conductance measurement. | |
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MedLine Citation:
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PMID: 22931376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a platform for sequence-specific nucleic acid (NA) detection utilizing a micropipet tapered to a 2 μm diameter pore and 3 μm diameter polystyrene beads to which uncharged peptide nucleic acid (PNA) probe molecules have been conjugated. As the target NAs hybridize to the complementary PNA-beads, the beads acquire negative charge and become electrophoretically mobile. An applied electric field guides these NA-PNA-beads toward the pipet tip, which they obstruct, leading to an indefinite, electrically detectable, partial blockade of the pore. In the presence of noncomplementary NA, even to the level of single base mismatch, permanent pore blockade is not seen. We show application of this platform to detection of the anthrax lethal factor sequence. |
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Authors:
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Leyla Esfandiari; Harold G Monbouquette; Jacob J Schmidt |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-09-12 |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 134 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-26 Completed Date: 2013-01-29 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 15880-6 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of California, Los Angeles, California, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA, Single-Stranded Electrophoretic Mobility Shift Assay Nucleic Acid Hybridization Peptide Nucleic Acids / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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R21 HG006157/HG/NHGRI NIH HHS; R21HG006157-01/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Single-Stranded; 0/Peptide Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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