Document Detail


Sequence-specific nucleic acid detection from binary pore conductance measurement.
MedLine Citation:
PMID:  22931376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a platform for sequence-specific nucleic acid (NA) detection utilizing a micropipet tapered to a 2 μm diameter pore and 3 μm diameter polystyrene beads to which uncharged peptide nucleic acid (PNA) probe molecules have been conjugated. As the target NAs hybridize to the complementary PNA-beads, the beads acquire negative charge and become electrophoretically mobile. An applied electric field guides these NA-PNA-beads toward the pipet tip, which they obstruct, leading to an indefinite, electrically detectable, partial blockade of the pore. In the presence of noncomplementary NA, even to the level of single base mismatch, permanent pore blockade is not seen. We show application of this platform to detection of the anthrax lethal factor sequence.
Authors:
Leyla Esfandiari; Harold G Monbouquette; Jacob J Schmidt
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-12
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  134     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-26     Completed Date:  2013-01-29     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15880-6     Citation Subset:  IM    
Affiliation:
Department of Bioengineering, University of California, Los Angeles, California, United States.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA, Single-Stranded
Electrophoretic Mobility Shift Assay
Nucleic Acid Hybridization
Peptide Nucleic Acids / analysis*
Grant Support
ID/Acronym/Agency:
R21 HG006157/HG/NHGRI NIH HHS; R21HG006157-01/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Peptide Nucleic Acids
Comments/Corrections

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