| Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. | |
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MedLine Citation:
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PMID: 14970398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells. |
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Authors:
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Gunter Meister; Markus Landthaler; Yair Dorsett; Thomas Tuschl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 10 ISSN: 1355-8382 ISO Abbreviation: RNA Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-02-18 Completed Date: 2004-03-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 544-50 Citation Subset: IM |
Affiliation:
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Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Genes, Reporter Hela Cells Humans MicroRNAs / metabolism* Oligoribonucleotides, Antisense / metabolism RNA Interference / physiology* RNA, Small Interfering / metabolism* Ribonucleoproteins / metabolism Transfection |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM068476-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MicroRNAs; 0/Oligoribonucleotides, Antisense; 0/RNA, Small Interfering; 0/Ribonucleoproteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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