Document Detail

Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing.
MedLine Citation:
PMID:  14970398     Owner:  NLM     Status:  MEDLINE    
A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.
Gunter Meister; Markus Landthaler; Yair Dorsett; Thomas Tuschl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  RNA (New York, N.Y.)     Volume:  10     ISSN:  1355-8382     ISO Abbreviation:  RNA     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-18     Completed Date:  2004-03-24     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9509184     Medline TA:  RNA     Country:  United States    
Other Details:
Languages:  eng     Pagination:  544-50     Citation Subset:  IM    
Laboratory of RNA Molecular Biology, The Rockefeller University, New York, New York 10021, USA.
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MeSH Terms
Genes, Reporter
HeLa Cells
MicroRNAs / metabolism*
Oligoribonucleotides, Antisense / metabolism
RNA Interference / physiology*
RNA, Small Interfering / metabolism*
Ribonucleoproteins / metabolism
Grant Support
R01 GM068476-01/GM/NIGMS NIH HHS
Reg. No./Substance:
0/MicroRNAs; 0/Oligoribonucleotides, Antisense; 0/RNA, Small Interfering; 0/Ribonucleoproteins

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