Document Detail


Sequence of neuron origin and neocortical laminar fate: relation to cell cycle of origin in the developing murine cerebral wall.
MedLine Citation:
PMID:  10575033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurons destined for each region of the neocortex are known to arise approximately in an "inside-to-outside" sequence from a pseudostratified ventricular epithelium (PVE). This sequence is initiated rostrolaterally and propagates caudomedially. Moreover, independently of location in the PVE, the neuronogenetic sequence in mouse is divisible into 11 cell cycles that occur over a 6 d period. Here we use a novel "birth hour" method that identifies small cohorts of neurons born during a single 2 hr period, i.e., 10-20% of a single cell cycle, which corresponds to approximately 1.5% of the 6 d neuronogenetic period. This method shows that neurons arising with the same cycle of the 11 cycle sequence in mouse have common laminar fates even if they arise from widely separated positions on the PVE (neurons of fields 1 and 40) and therefore arise at different embryonic times. Even at this high level of temporal resolution, simultaneously arising cells occupy more than one cortical layer, and there is substantial overlap in the distributions of cells arising with successive cycles. We demonstrate additionally that the laminar representation of cells arising with a given cycle is little if at all modified over the early postnatal interval of histogenetic cell death. We infer from these findings that cell cycle is a neuronogenetic counting mechanism and that this counting mechanism is integral to subsequent processes that determine cortical laminar fate.
Authors:
T Takahashi; T Goto; S Miyama; R S Nowakowski; V S Caviness
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  19     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  1999-12-17     Completed Date:  1999-12-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10357-71     Citation Subset:  IM; S    
Affiliation:
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA. tata@med.keio.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology
Animals
Animals, Newborn / anatomy & histology,  growth & development
Brain / embryology*,  growth & development
Cell Cycle / physiology
Embryo, Mammalian / cytology,  physiology
Embryonic and Fetal Development / physiology
Mice
Mice, Inbred Strains
Neocortex / cytology*
Neurons / cytology*,  physiology*
Grant Support
ID/Acronym/Agency:
NS12005/NS/NINDS NIH HHS; NS33433/NS/NINDS NIH HHS
Investigator
Investigator/Affiliation:
R S Nowakowski / U Med & Dent NJ, Piscataway

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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