Document Detail


Sequence homology between the RNA of Mason-Pfizer monkey virus and the RNA of human malignant breast tumors.
MedLine Citation:
PMID:  4216026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Radioactive DNA ([(3)H]cDNA) complementary to the RNA of Mason-Pfizer monkey virus was used in molecular hybridization experiments to demonstrate sequence homology between its viral RNA and RNA of human malignant breast tumors. Hybridization products were analyzed by cesium sulfate equilibrium density centrifugation and hydroxylapatite chromatography. Seven of ten human malignant breast tumors tested contained virus-related RNA when assayed by hydroxylapatite chromatography, whereas no sequence homology was found in RNA from 11 human benign breast tumors or from RNA of normal human spleen, liver, or kidney. Hybridizations between [(3)H]cDNA of the monkey virus and human breast adenocarcinoma RNA showed a C(r)t((1/2)) of about 2 x 10(4). The fidelity of the hybrids formed was indicated by a T(m) of 80.5 degrees .
Authors:
D Colcher; S Spiegelman; J Schlom
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 Dec 
Date Detail:
Created Date:  1975-03-10     Completed Date:  1975-03-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4975-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / analysis*
Animals
Base Sequence
Breast Neoplasms / analysis*
Centrifugation, Density Gradient
Chromatography
DNA, Viral
Female
Haplorhini
Humans
Hydroxyapatites
Nucleic Acid Hybridization
Oncogenic Viruses / analysis*
RNA Viruses / analysis
RNA, Neoplasm / analysis*
RNA, Viral / analysis*,  biosynthesis
Templates, Genetic
Tritium
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Hydroxyapatites; 0/RNA, Neoplasm; 0/RNA, Viral; 10028-17-8/Tritium
Comments/Corrections

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