| Sequence heterogeneity and polymorphic gene arrangements of the Leishmania guyanensis gp63 genes. | |
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MedLine Citation:
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PMID: 7908120 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Leishmania GP63 major surface protein gene family encodes multiple isoforms which differ predominantly in the carboxyterminal region. We have isolated 4 full-length gp63 cDNA clones derived from stationary-phase promastigote RNA of a cloned isolate of Leishmania guyanensis, a member of the braziliensis complex. These genes, along with the previously published L. guyanensis gp63 gene sequence [15], appeared to be mosaics of different combinations of 5' and 3' untranslated regions and sequences encoding the propeptide, internal, and C-terminal regions of GP63. The predicted L. guyanensis GP63 isoforms shared as little as 55% sequence identity, comparable to the inter-species diversity of GP63. The genomic organization of gp63 genes in L. guyanensis is highly complex: there are at least 4 distinct polymorphic forms of tandemly linked gene clusters, with intra-gene cluster variation in gene sequence and in the number of gene repeats. Southern blot analysis suggested that the arrangement of gp63 genes in this L. guyanensis isolate did not differ from that in the parental lines. |
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Authors:
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H B Steinkraus; J M Greer; D C Stephenson; P J Langer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and biochemical parasitology Volume: 62 ISSN: 0166-6851 ISO Abbreviation: Mol. Biochem. Parasitol. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-04-22 Completed Date: 1994-04-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8006324 Medline TA: Mol Biochem Parasitol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 173-85 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, University of Wyoming, Laramie 82071-3944. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L16776; L16777; L16778; L16779 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cosmids DNA, Protozoan Genes, Protozoan Leishmania guyanensis / genetics* Metalloendopeptidases / genetics* Molecular Sequence Data Multigene Family* Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| Grant Support | |
ID/Acronym/Agency:
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AI-25031/AI/NIAID NIH HHS; S07RR07157-17/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/glycoprotein gp63, Leishmania |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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