Document Detail


Sequence and expression of human hair keratin genes.
MedLine Citation:
PMID:  7528047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal hair growth and differentiation requires co-ordinate expression of many hair specific structural protein genes. It has been established that one of the 4 major groups of hair structural proteins, low-sulphur hair keratins, belongs to the intermediate filament (IF) multigene family. Hair keratin IF proteins differ from those of other epithelia as they contain cysteine-rich terminal domains allowing more extensive disulphide bonding to the high-sulphur hair matrix proteins. Until recently, little information concerning the primary sequence of hair keratins was available but cloning of some mouse hair and sheep wool keratins has now been reported. Using these sequences, we have polymerase chain reaction (PCR) amplified genomic fragments of human hair-specific keratin IF genes and isolated cosmid clones containing full length genes. We have sequenced part of these genes and studied their expression in human hair follicles. Hair specific keratin fragments were amplified from placental gDNA by PCR primed with synthetic oligonucleotides. Fragments were cloned and sequenced after ligation into pGEM-3Z and labelled riboprobes were generated for in situ hybridization on human skin sections. A human cosmid library was screened with PCR fragments and clones encoding human hair keratin genes were characterised by southern hybridization and sequencing. The type I human hair-specific keratin clones obtained (HaKA1-b2, 386 bp; hHaKA1-XH1, 1202 bp) encoded 2B helix, C-terminal and 3'nc regions and were 65% homologous to mouse sequences. The type II hair keratin clone (hHaKB2-1, 829 bp) also encoded 2B helix and C-terminal regions and was 95% homologous to mouse. In situ hybridization on human skin sections showed a specific reaction with precortical cells of the hair follicle. One human cosmid clone, isolated with the hHaKB2-1 probe, contained two type II hair keratin genes about 7 kb apart, each of which had 9 exons spanning approximately 6 kb. The coding sequences were homologous to mouse cDNA (77-88%). These human hair-specific keratin clones are useful molecular tools for studies of hair differentiation.
Authors:
P E Bowden; S Hainey; G Parker; M B Hodgins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dermatological science     Volume:  7 Suppl     ISSN:  0923-1811     ISO Abbreviation:  J. Dermatol. Sci.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1995-01-24     Completed Date:  1995-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9011485     Medline TA:  J Dermatol Sci     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  S152-63     Citation Subset:  IM    
Affiliation:
Department of Dermatology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S75796;  S75797
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cosmids
DNA / genetics
Gene Expression
Hair / growth & development,  metabolism*
Humans
In Situ Hybridization
Keratins / chemistry,  classification,  genetics*
Mice
Molecular Sequence Data
Molecular Structure
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Sheep
Species Specificity
Chemical
Reg. No./Substance:
68238-35-7/Keratins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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