| Sequence diversity in the 5' untranslated region of rabbit muscle phosphofructokinase mRNA. | |
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MedLine Citation:
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PMID: 2143897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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DNA sequences of two full-length rabbit muscle phosphofructokinase (RMPFK) cDNAs (A and B) show identical coding sequence but heterogeneous 5' untranslated regions. cDNA-A is formed by removal of a 1.7 kb upstream intron while cDNA-B retains the 3' region of this intron. A 2.8 kb upstream sequence of RMPFK gene contains several features characteristic of housekeeping genes: high GC content (67%) at its 5' end, with 50 CpG sites; five Sp1 sites; and no functional TATA box. Comparison of the 5' sequences of the two RMPFK cDNAs and three human muscle PFK cDNAs (Nakajima, H., et al., (1990) Biochem. Biophys. Res. Com. 166, 637) suggests that a single splicing event occurs involving different splicing donor sites but the same splicing acceptor site, resulting in diversity in the upstream sequence. These observations suggest that transcription of muscle PFK gene may start at multiple sites, another feature of housekeeping genes. |
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Authors:
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J Li; Z Chen; L Lu; M Byrnes; S H Chang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 170 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-09-27 Completed Date: 1990-09-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1056-60 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Louisiana State University, Baton Rouge 70803. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M57452 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA / analysis* Genomic Library Humans Molecular Sequence Data Muscles / enzymology* Phosphofructokinase-1 / genetics* RNA, Messenger / analysis* Rabbits Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic* |
| Grant Support | |
ID/Acronym/Agency:
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DK 31676/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9007-49-2/DNA; EC 2.7.1.11/Phosphofructokinase-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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