Document Detail


Sequence of the coding regions from the 3.0 kb and 3.9 kb mRNA. Subgenomic species from a virulent isolate of transmissible gastroenteritis virus.
MedLine Citation:
PMID:  2546515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA clones covering the genome region from the 3' end of the peplomer gene to the start of the integral membrane protein gene. The nucleotide sequence of this area was determined using clone pTG11 and a previously reported cDNA clone pTG22. Three open reading frames (ORFs) were identified encoding putative polypeptides of relative molecular masses (Mr) 6,600, 27,600, and 9,200. The sequence encoding the Mr 9,200 polypeptide was found to be present on the "unique" 5' region of the 3.0 kb mRNA species whereas the other two ORFs mapped on the 3.9 kb mRNA species. Differences between the ORFs from this strain of TGEV and those from a previously reported avirulent strain of TGEV were compared.
Authors:
P Britton; C Lopez Otin; J Martin Alonso; F Parra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  105     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1989  
Date Detail:
Created Date:  1989-08-16     Completed Date:  1989-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  165-78     Citation Subset:  IM    
Affiliation:
Division of Microbiology, A.F.R.C. Institute for Animal Health, Compton Laboratory, Newbury, Berks, U.K.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X14551
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Blotting, Northern
Cloning, Molecular
Coronaviridae / genetics*
DNA, Viral / genetics
Molecular Weight
Peptides / genetics
RNA, Messenger / genetics*
RNA, Viral / genetics*
Species Specificity
Transmissible gastroenteritis virus / classification,  genetics*,  pathogenicity
Viral Proteins / genetics
Virulence
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Peptides; 0/RNA, Messenger; 0/RNA, Viral; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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