Document Detail

Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA.
Jump to Full Text
MedLine Citation:
PMID:  8759015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The PCR amplification of tetranucleotide short tandem repeat (STR) loci typically produces a minor product band 4 bp shorter than the corresponding main allele band; this is referred to as the stutter band. Sequence analysis of the main and stutter bands for two sample alleles of the STR locus vWA reveals that the stutter band lacks one repeat unit relative to the main allele. Sequencing results also indicate that the number and location of the different 4 bp repeat units vary between samples containing a typical verses low proportion of stutter product. The results also suggest that the proportion of stutter product relative to the main allele increases as the number of uninterrupted core repeat units increases. The sequence analysis and results obtained using various DNA polymerases appear to support the slipped strand displacement model as a potential explanation for how these stutter products are generated.
Authors:
P S Walsh; N J Fildes; R Reynolds
Related Documents :
7698505 - Association of the prohormone convertase 2 gene (pcsk2) on chromosome 20 with niddm in ...
19789645 - Decreased rate of evolution in y chromosome str loci of increased size of the repeat unit.
15468075 - Huntington's disease-like 2 (hdl2) in north america and japan.
2086355 - Characterization and distribution of an interspersed repeated nucleotide sequence from ...
22580585 - The xx sex chromosome complement in mice is associated with increased spontaneous lupus...
25112205 - Mapping resistance genes for oculimacula acuformis in aegilops longissima.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  24     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-09-17     Completed Date:  1996-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2807-12     Citation Subset:  IM    
Affiliation:
Human Identity Group, Roche Molecular Systems Inc., Alameda, CA 94501, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alleles
Base Sequence
DNA-Directed DNA Polymerase / metabolism
Genetic Markers
Microsatellite Repeats*
Polymerase Chain Reaction
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Genetic Markers; EC 2.7.7.7/DNA-Directed DNA Polymerase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
Download PDF

Print publication date: Day: 15 Month: 7 Year: 1996
Volume: 24 Issue: 14
First Page: 2807 Last Page: 2812
ID: 146018
PubMed Id: 8759015
Publisher Item Identifier: 6j0077

Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA.
P S Walsh
N J Fildes
R Reynolds
Human Identity Group, Roche Molecular Systems Inc., Alameda, CA 94501, USA.


Article Categories:
  • Research Article


Previous Document:  Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur...
Next Document:  E2A basic-helix-loop-helix transcription factors are negatively regulated by serum growth factors an...