Document Detail

Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle.
MedLine Citation:
PMID:  15480739     Owner:  NLM     Status:  MEDLINE    
Various cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle.
Shinichiro Hayashi; Hisashi Aso; Kouichi Watanabe; Hidetoshi Nara; Michael T Rose; Shyuichi Ohwada; Takahiro Yamaguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-05
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  122     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2005-05-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  427-34     Citation Subset:  IM    
Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
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MeSH Terms
Cell Proliferation / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Hepatocyte Growth Factor / metabolism*,  pharmacology
Insulin-Like Growth Factor I / metabolism*
Insulin-Like Growth Factor II / metabolism*
Muscle Fibers, Skeletal / drug effects,  metabolism
Muscle, Skeletal / cytology,  physiology*
Myoblasts / drug effects,  metabolism
RNA, Messenger / metabolism
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Reg. No./Substance:
0/RNA, Messenger; 0/Recombinant Proteins; 67256-21-7/Hepatocyte Growth Factor; 67763-96-6/Insulin-Like Growth Factor I; 67763-97-7/Insulin-Like Growth Factor II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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