Document Detail


Septins enforce morphogenetic events during sexual reproduction and contribute to virulence of Cryptococcus neoformans.
MedLine Citation:
PMID:  19943902     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Septins are conserved, cytoskeletal GTPases that contribute to cytokinesis, exocytosis, cell surface organization and vesicle fusion by mechanisms that are poorly understood. Roles of septins in morphogenesis and virulence of a human pathogen and basidiomycetous yeast Cryptococcus neoformans were investigated. In contrast to a well-established paradigm in S. cerevisiae, Cdc3 and Cdc12 septin homologues are dispensable for growth in C. neoformans yeast cells at 24 degrees C but are essential at 37 degrees C. In a bilateral cross between septin mutants, cells fuse but the resulting hyphae exhibit morphological abnormalities, including lack of properly fused specialized clamp cells and failure to produce spores. Interestingly, post-mating hyphae of the septin mutants have a defect in nuclear distribution. Thus, septins are essential for the development of spores, clamp cell fusion and also play a specific role in nuclear dynamics in hyphae. In the post-mating hyphae the septins localize to discrete sites in clamp connections, to the septa and the bases of the initial emerging spores. Strains lacking CDC3 or CDC12 exhibit significantly reduced virulence in a Galleria mellonella model of infection. Thus, C. neoformans septins are vital to morphology of the hyphae and contribute to virulence.
Authors:
Lukasz Kozubowski; Joseph Heitman
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-11-25
Journal Detail:
Title:  Molecular microbiology     Volume:  75     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-05-06     Completed Date:  2010-07-27     Revised Date:  2013-07-26    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  658-75     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Cryptococcus neoformans / cytology*,  pathogenicity*,  ultrastructure
Cytoskeletal Proteins / analysis,  classification,  physiology*
Fungal Proteins / analysis,  classification,  physiology*
GTP Phosphohydrolases / analysis,  classification,  physiology*
Humans
Hyphae / chemistry,  cytology,  growth & development
Morphogenesis
Saccharomyces cerevisiae Proteins / classification
Virulence
Grant Support
ID/Acronym/Agency:
R01 AI042159/AI/NIAID NIH HHS; R01 AI39115/AI/NIAID NIH HHS; R01 AI42159/AI/NIAID NIH HHS; R37 AI039115/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/Fungal Proteins; 0/Saccharomyces cerevisiae Proteins; EC 3.6.1.-/GTP Phosphohydrolases
Comments/Corrections

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