Document Detail

Septin9 is involved in septin filament formation and cellular stability.
MedLine Citation:
PMID:  21824004     Owner:  NLM     Status:  In-Data-Review    
Abstract Septin9 (Sept9) is a member of the filament-forming septin family of structural proteins and is associated with a variety of cancers and with hereditary neuralgic amyotrophy. We have generated mice with constitutive and conditional Sept9 knockout alleles. Homozygous deletion of Sept9 results in embryonic lethality around day 10 of gestation whereas mice homozygous for the conditional allele develop normally. Here we report the consequences of homozygous loss of Sept9 in immortalized murine embryonic fibroblasts. Proliferation rate was not changed but cells without Sept9 had an altered morphology compared to normal cells, particularly under low serum stress. Abnormal, fragmented, and multiple nuclei were more frequent in cells without Sept9. Cell migration, as measured by gap-filling and filter-invasion assays, was impaired, but individual cells did not move less than wild-type cells. Sept9 knockout cells showed a reduced resistance to hypo-osmotic stress. Stress fiber and vinculin staining at focal adhesion points was less prominent. Long septin filaments stained for Sept7 disappeared. Instead, staining was found in short, often curved filaments and rings. Furthermore, Sept7 was no longer localized to the mitotic spindle. Together, these data reveal the importance of Sept9 for septin filament formation and general cell stability.
Annette Füchtbauer; Louise B Lassen; Astrid B Jensen; Jennifer Howard; Adán de Salas Quiroga; Søren Warming; Annette B Sørensen; Finn S Pedersen; Ernst-Martin Füchtbauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological chemistry     Volume:  392     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  769-77     Citation Subset:  IM    
University of Aarhus, Molecular Biology, C.F. Møllers Allé 3, DK-Aarhus 8000, Denmark.
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