Document Detail


Septin1 is required for spindle assembly and chromosome congression in mouse oocytes.
MedLine Citation:
PMID:  21932310     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The bipolar spindle is a complex molecular machinery that drives chromosome congression and segregation. During meiosis in the mouse multiple microtubule organizing centers aggregate to form a bipolar intermediate followed by elongation and establishment of the barrel-shaped acentriolar meiotic spindle. Previous studies have shown that septin1 is localized to spindle poles in mitosis, suggesting its possible involvement in spindle assembly. We, therefore, asked whether perturbation of septin1 will impair the process of spindle assembly and investigated localization and function during mouse oocyte meiotic maturation. Septin1 was localized to the spindle at metaphase and at the midbody during cytokinesis. Disruption of septin1 function using siRNA caused a decrease in PBE and extensive spindle defects. Moreover, the process of chromosome congression was impaired. However, septin1 depletion did not cause aneuploidy in oocyte with an extruded polar body. Taken together, our results show that septin1 is a key player in spindle assembly and chromosome congression in mouse meiosis. Developmental Dynamics 240:2281-2289, 2011. © 2011 Wiley-Liss, Inc.
Authors:
Jinliang Zhu; Shu-Tao Qi; Ya-Peng Wang; Zhen-Bo Wang; Ying-Chun Ouyang; Yi Hou; Heide Schatten; Qing-Yuan Sun
Publication Detail:
Type:  Journal Article     Date:  2011-08-26
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  240     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2281-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; Graduate School, Chinese Academy of Sciences, Beijing, China.
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