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Separation of urinary oestrogen sulphates from oestrogen glucosiduronates on diethylaminoethyl-Sephadex columns.
MedLine Citation:
PMID:  16742529     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
1. Gradient elution (sodium chloride concentration gradient) on DEAE-Sephadex columns is used to separate urinary oestrogen conjugates of pregnancy urine. Changes in the shape of the gradient alter the chromatograms in a predictable manner so that the dispersion of peaks can be modified at will. 2. Suitable choice of the gradient results in the separation from each other of oestrogen sulphates, oestrogen 16(or 17)-glucosiduronates, oestrogen 3-glucosiduronates and free oestrogens. 3. Evidence for the presence of oestriol 3-sulphate, oestrone 3-sulphate, 17beta-oestradiol 3-sulphate, 16-oxo-17beta-oestradiol 3-sulphate and oestriol 16(or 17)-sulphate in the sulphate fraction of DEAE-Sephadex chromatograms of pregnancy urine is provided.
Authors:
R Hähnel; M G Rahman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  105     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1967 Dec 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-06-25     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  1047-53     Citation Subset:  -    
Affiliation:
Queen Elizabeth II Coronation Gift Fund and University Department of Obstetrics and Gynaecology, King Edward Memorial Hospital for Women, Subiaco, and Department of Biochemistry, University of Western Australia, Nedlands, Western Australia.
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