Document Detail


Separation of sperm and epithelial cells in a microfabricated device: potential application to forensic analysis of sexual assault evidence.
MedLine Citation:
PMID:  15679339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Forensic DNA analysis of sexual assault evidence requires separation of DNA from epithelial (victim) and sperm (perpetrator) cells. The conventional method used by crime laboratories, which is termed "differential extraction", is a time-consuming process. To supplant the conventional process, separation of sperm from a biological mixture containing epithelial cells has been demonstrated on a microfluidic device. This separation utilizes the differential physical properties of the cells that result in settling of the epithelial cells to the bottom of the inlet reservoir and subsequent adherence to the glass substrate. As a result, low flow rates can be used to separate the sperm cells from the epithelial cell-containing biological mixture. Following cell separation on the microdevice, DNA extraction, amplification, and separation were performed using conventional laboratory methods, showing that the cell separation product in the outlet reservoir was of male origin. The reported cell separation has the potential to impact the forensic DNA analysis backlog of sexual assault cases by circumventing the time-consuming conventional differential extraction procedure.
Authors:
Katie M Horsman; Susan L R Barker; Jerome P Ferrance; Kymberly A Forrest; Katherine A Koen; James P Landers
Related Documents :
25170899 - Cell surface profiling using high-throughput flow cytometry: a platform for biomarker d...
25268519 - Synthesis and characterization of novel 2-amino-chromene-nitriles that target bcl-2 in ...
2329479 - Study of the lens epithelial cell density in cataractous eyes operated on with extracap...
18513459 - Improved bioluminescent assay of somatic cell counts in raw milk.
25190089 - Fast and continuous microorganism detection using aptamer-conjugated fluorescent nanopa...
25471819 - Activation of epidermal growth factor receptor is required for chlamydia trachomatis de...
8663289 - Chinese hamster ovary cells expressing a cell surface-anchored form of hepatic lipase. ...
9148769 - Antibody selection against cd52 produces a paroxysmal nocturnal haemoglobinuria phenoty...
24221419 - Cellulose-microfibril-orienting mechanisms in plant cells walls.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  77     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-31     Completed Date:  2005-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  742-9     Citation Subset:  IM    
Affiliation:
Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA 22904, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Separation / instrumentation,  methods*
DNA / analysis,  genetics
Epithelial Cells / cytology*
Equipment Design
Forensic Medicine*
Genetic Markers
Humans
Male
Nanotechnology
Semen / cytology
Sex Offenses*
Spermatozoa / cytology*
Chemical
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Direct molecular imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by ma...
Next Document:  Importance of solubility in the sample preparation of poly(ethylene terephthalate) for MALDI TOFMS.