Document Detail


Separation of sperm and epithelial cells in a microfabricated device: potential application to forensic analysis of sexual assault evidence.
MedLine Citation:
PMID:  15679339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Forensic DNA analysis of sexual assault evidence requires separation of DNA from epithelial (victim) and sperm (perpetrator) cells. The conventional method used by crime laboratories, which is termed "differential extraction", is a time-consuming process. To supplant the conventional process, separation of sperm from a biological mixture containing epithelial cells has been demonstrated on a microfluidic device. This separation utilizes the differential physical properties of the cells that result in settling of the epithelial cells to the bottom of the inlet reservoir and subsequent adherence to the glass substrate. As a result, low flow rates can be used to separate the sperm cells from the epithelial cell-containing biological mixture. Following cell separation on the microdevice, DNA extraction, amplification, and separation were performed using conventional laboratory methods, showing that the cell separation product in the outlet reservoir was of male origin. The reported cell separation has the potential to impact the forensic DNA analysis backlog of sexual assault cases by circumventing the time-consuming conventional differential extraction procedure.
Authors:
Katie M Horsman; Susan L R Barker; Jerome P Ferrance; Kymberly A Forrest; Katherine A Koen; James P Landers
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  77     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-31     Completed Date:  2005-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  742-9     Citation Subset:  IM    
Affiliation:
Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA 22904, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / instrumentation,  methods*
DNA / analysis,  genetics
Epithelial Cells / cytology*
Equipment Design
Forensic Medicine*
Genetic Markers
Humans
Male
Nanotechnology
Semen / cytology
Sex Offenses*
Spermatozoa / cytology*
Chemical
Reg. No./Substance:
0/Genetic Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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