Document Detail


Separation of resting and proliferating granulocytic precursors.
MedLine Citation:
PMID:  521619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have obtained cells in various stages of granulocytic development by a combination of isopycnic separation and electronic cell sorting. Not only were immature cells (blast cells, promyelocytes and myelocytes) separated from mature cells (bands and polys), but the immature cells were separated into proliferating (S + G2 + M) and resting (Go/G1) compartments of the cell cycle. This permits the study of the morphological and biochemical changes associated with development apart from those changes associated with proliferation.
Authors:
W M Grogan; R B Scott; J M Collins
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  27     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-03-27     Completed Date:  1980-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1638-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells
Cell Cycle*
Cell Separation / methods*
Centrifugation, Isopycnic
DNA / analysis
Granulocytes / analysis,  cytology*
Hematopoiesis
Interphase*
Male
Mitosis*
Rabbits
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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