Document Detail


Separation and quantitation of fatty acids by high-performance liquid chromatography.
MedLine Citation:
PMID:  3700549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To facilitate the determination of the fatty acid composition of tissues and the investigation of fatty acid metabolism, we developed a method for the rapid separation by high-performance liquid chromatography and quantitation (by ultraviolet light absorption) of p-bromophenyl esters of fatty acids which vary in chain length from 10 to 22 carbon atoms. The utility of the method was demonstrated by evaluating the fatty acid composition of human uterine decidua vera tissue and human endometrial stromal cells that are maintained in monolayer culture.
Authors:
K Korte; K R Chien; M L Casey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of chromatography     Volume:  375     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-06-03     Completed Date:  1986-06-03     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  225-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Endometrium / analysis
Fatty Acids / isolation & purification*
Female
Humans
Spectrophotometry, Ultraviolet
Grant Support
ID/Acronym/Agency:
2-P01-AG00306/AG/NIA NIH HHS; 5-P50-HD11149/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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