Document Detail


Separation and purification of rutin and acaciin from the chinese medicinal herb Herba Cirsii by combination of macroporous absorption resin and high-speed counter-current chromatography.
MedLine Citation:
PMID:  19476696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of rutin and acaciin from the Chinese medicinal herb Herba Cirsii was successfully established. The crude extracts obtained from Herba Cirsii by water under reflux were subjected to a macroporous resin column and eluted with 10% and 60% ethanol, respectively. The fraction of 60% ethanol was used as the sample for HSCCC separation of rutin and acaciin. The two-phase solvent system used for the separation was ethyl acetate-n-butanol-water (5:1.5:5, v/v) and the upper phase was used as the stationary phase. Rutin (25.2 mg) and acaciin (21.8 mg) with a purity of 99.2% and 99.6%, respectively, were purified successfully from 60 mg of sample. The chemical structures of rutin and acaciin were identified by 1H-NMR and 13C-NMR.
Authors:
Li Meng; Renmin Liu; Ailing Sun; Sujuan Wu; Ningnig Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  47     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:    2009 May-Jun
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  329-32     Citation Subset:  IM    
Affiliation:
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Chromatography, High Pressure Liquid / methods
Countercurrent Distribution / methods*
Drugs, Chinese Herbal
Glycosides / isolation & purification*
Herbal Medicine*
Magnetic Resonance Spectroscopy
Rutin / isolation & purification*
Chemical
Reg. No./Substance:
0/Drugs, Chinese Herbal; 0/Glycosides; 153-18-4/Rutin; 480-36-4/linarin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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